Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.
Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LABS.
MW: 527.52 g/mol
Storage/Handling: Store desiccated at -20°C.
PubChem Chemical ID: 35766
|Storage/Handling||Store desiccated at -20°C.|
Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola - method by Flowers and Vaillancourt (2005)
Developing a Titration Kill Curve for cell cultures with G418, Hygromycin and Puromycin
Hygromycin B Cell Culture and Plate Preparation for bacteria, plant and mammalian cells and yeast.
Protocol for yeast plate preparation and screening of transformed yeast.