Hygromycin B EZ Pak™ for 50 mg/mL Solution
Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.
Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.
The Hygromycin B EZ Pak™ is the fastest and easiest way to make a set amount of sterile hygromycin B solution. The kit includes pre-weighed hygromycin B powder, a sterile filter and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio hygromycin B and the sterile solution is ready for tissue culture, bacterial media or any other appropriate use.
The 25 ml, 50 ml, and 100 ml pack sizes require a vacuum manifold for easy vacuum filtering, while the 10 ml pack size comes with a syringe filter.
Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola - method by Flowers and Vaillancourt (2005)
Hygromycin B Cell Culture and Plate Preparation for bacteria, plant and mammalian cells and yeast.