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    Affinity reagents and affinity-based tools utilize complex networks of specific interactions between various molecules for use in the separation, purification or analysis of proteins. Affinity purification requires an affinity ligand to be coupled to a matrix, which allows capture of the intended product from a complex mixture. Gold Biotechnology’s affinity reagents are activated beads or preactivated resins for use in coupling of affinity ligands and immobilization. Our affinity beads are specifically engineered to meet your specialized needs, whether it be laboratory scale experiments or industry scale separations. GoldBio’s agarose beads are conveniently cross-linked to include any of the following: aminoethyl or glyoxal groups, glutathione S-transferase, Protein A, Protein G, streptavidin and His-tag metal ions such as nickel, cobalt, copper or zinc.

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    Affinity reagents and affinity-based tools utilize complex networks of specific interactions between various molecules for use in the separation, purification or analysis of proteins. Affinity purification requires an affinity ligand to be coupled to a matrix, which allows capture of the intended product from a complex mixture. Gold Biotechnology’s affinity reagents are activated beads or preactivated resins for use in coupling of affinity ligands and immobilization. Our affinity beads are specifically engineered to meet your specialized needs, whether it be laboratory scale experiments or industry scale separations. GoldBio’s agarose beads are conveniently cross-linked to include any of the following: aminoethyl or glyoxal groups, glutathione S-transferase, Protein A, Protein G, streptavidin and His-tag metal ions such as nickel, cobalt, copper or zinc.

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  • object(anvilstl\commercio\models\Category)#440 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(8) ["root"]=> int(1) ["lft"]=> int(74) ["rgt"]=> int(89) ["level"]=> int(3) ["name"]=> string(20) "His-tag Purification" ["url_name"]=> string(20) "his-tag-purification" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(834) "His-tag purification is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and chemistry that need to be separated. GoldBio's IDA cross-linked agarose resins consist of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. These resins are loaded with a divalent metal ions (such as nickel, cobalt, zinc or copper) in either high density or low density affinities to meet your specific research requirements. Copper ions have high affinity (binding strength) but low specificity (selectivity); cobalt ions have low affinity but have high specificity; while nickel and zinc combine a mid-ranged affinity and specificity for most general purposes.
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NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeDelete" } [1]=> NULL } } ["afterDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#443 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#443 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }
  • object(anvilstl\commercio\models\Category)#444 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(194) ["root"]=> int(1) ["lft"]=> int(3) ["rgt"]=> int(4) ["level"]=> int(2) ["name"]=> string(10) "A_Products" ["url_name"]=> string(10) "a-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(194) ["root"]=> int(1) ["lft"]=> int(3) ["rgt"]=> 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object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeInsert" } [1]=> NULL } } ["afterInsert"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterInsert" } [1]=> NULL } } ["beforeUpdate"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeUpdate" } [1]=> NULL } } ["afterUpdate"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterUpdate" } [1]=> NULL } } ["beforeDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeDelete" } [1]=> NULL } } ["afterDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }
  • object(anvilstl\commercio\models\Category)#446 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(229) ["root"]=> int(1) ["lft"]=> int(25) ["rgt"]=> int(26) ["level"]=> int(2) ["name"]=> string(10) "N_Products" ["url_name"]=> string(10) "n-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(229) ["root"]=> int(1) ["lft"]=> int(25) 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{ ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeDelete" } [1]=> NULL } } ["afterDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }
  • object(anvilstl\commercio\models\Category)#448 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(83) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Agarose-Resins.jpg" ["medium"]=> string(84) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Agarose-Resins.jpg" ["large"]=> string(83) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Agarose-Resins.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(83) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Agarose-Resins.jpg" ["medium"]=> string(84) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Agarose-Resins.jpg" ["large"]=> string(83) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Agarose-Resins.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(242) ["root"]=> int(393) ["lft"]=> int(54) ["rgt"]=> int(55) ["level"]=> int(1) ["name"]=> string(20) "Agarose Resins (old)" ["url_name"]=> string(15) "agarose-resins2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(846) "

    Agarose resins possess physical characteristics which make them a cornerstone in the analytical field due to their ability to separate and purify molecules depending on the application. These resins can be used to manipulate proteins and amino acid binding to suit desirable outcomes. In His-Tag and size-exclusion chromatography, resins are used to help isolate specific proteins from undesirable ones for further applications. GST chromatography resins can be used to bind together DNA coding sequences. Protein A & G resins are also help bind immunoglobulins. For industrial scale applications, high-throughput chromatography resin allows for high flow rates without compromising binding capacity. GoldBio provides these high-quality chromatography products and the necessary equipment to design and perform separations for a low cost.

    " ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(23) ["banner_image_id"]=> int(0) ["meta_description"]=> string(153) "Agarose resins help isolate and separate molecules for various applications. GoldBio has low priced, high quality resins for separation and purification." ["meta_keywords"]=> string(72) "agarose resin, his-tag, histag, agarose beads, gst, protein a, protein g" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> string(21) "rgba(160, 221, 94, 1)" } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(242) ["root"]=> int(393) ["lft"]=> int(54) ["rgt"]=> int(55) ["level"]=> int(1) ["name"]=> string(20) "Agarose Resins (old)" ["url_name"]=> string(15) "agarose-resins2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(846) "

    Agarose resins possess physical characteristics which make them a cornerstone in the analytical field due to their ability to separate and purify molecules depending on the application. These resins can be used to manipulate proteins and amino acid binding to suit desirable outcomes. In His-Tag and size-exclusion chromatography, resins are used to help isolate specific proteins from undesirable ones for further applications. GST chromatography resins can be used to bind together DNA coding sequences. Protein A & G resins are also help bind immunoglobulins. For industrial scale applications, high-throughput chromatography resin allows for high flow rates without compromising binding capacity. GoldBio provides these high-quality chromatography products and the necessary equipment to design and perform separations for a low cost.

    " ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(23) ["banner_image_id"]=> int(0) ["meta_description"]=> string(153) "Agarose resins help isolate and separate molecules for various applications. GoldBio has low priced, high quality resins for separation and purification." 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  • Nickel Agarose Beads (Low Density)

    Product Description

    Low Density Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >12 mg/ml with approximately 60 kDa protein. It works very well with the His-Tag Buffer Set.

    IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

    Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

    Nickel agarose resin is suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.

    Storage/Handling: Store at 4°C. Do NOT freeze.

    Note: Some discoloration of the Nickel IDA resin has been observed in reaction with low levels of reducing agents such as DTT. However, the resin remains fully functional even at concentrations as high as 5mM DTT.

    Product Specifications

    Catalog ID H-321
    Storage/Handling store at 4°C. Do NOT freeze.

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    $9.99 Ground shipping (In continental US only.)

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