Agarose resins possess physical characteristics which make them a cornerstone in the analytical field due to their ability to separate and purify molecules depending on the application. These resins can be used to manipulate proteins and amino acid binding to suit desirable outcomes. In His-Tag and size-exclusion chromatography, resins are used to help isolate specific proteins from undesirable ones for further applications. GST chromatography resins can be used to bind together DNA coding sequences. Protein A & G resins are also help bind immunoglobulins. For industrial scale applications, high-throughput chromatography resin allows for high flow rates without compromising binding capacity. GoldBio provides these high-quality chromatography products and the necessary equipment to design and perform separations for a low cost.
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string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }GoldBio's Immobilized Enzyme Resins comprise a convenient method for digestion without the hassle of cleaning out the leftover enzyme after the reaction is finished! These enzymes are linked either to 4% or 6% agarose beads, which makes them easier to recycle and use again, if desired. With immobilized enzymes, the enzymatic rate of the reaction can be adjusted simply by changing the flow through rate and the amount of undesired protein loss through dissolving is also reduced. GoldBio additionally offers a wide variety of affinity purification agarose resins for the downstream applications of your digested products...everything you need for your experiment, at the prices you know are best!
GoldBio's Immobilized Enzyme Resins comprise a convenient method for digestion without the hassle of cleaning out the leftover enzyme after the reaction is finished! These enzymes are linked either to 4% or 6% agarose beads, which makes them easier to recycle and use again, if desired. With immobilized enzymes, the enzymatic rate of the reaction can be adjusted simply by changing the flow through rate and the amount of undesired protein loss through dissolving is also reduced. GoldBio additionally offers a wide variety of affinity purification agarose resins for the downstream applications of your digested products...everything you need for your experiment, at the prices you know are best!
Immobilized Pepsin Agarose Resin
Product Description
Pepsin, one of the principal digestion enzymes, breaks down proteins in to smaller peptides. It is most efficient in cleaving the peptide bonds between hydrophobic and aromatic amino acids such as phenylalanine, tryptophan and tyrosine. Pepsin readily cleaves immunoglobulin G (IgG) molecules, generating F(ab’)2 fragments and small Fc peptide fragments. The smaller Fc fragments can be easily removed via gel filtration while the F(ab’)2 fragments can then be purified from any remaining, undigested IgG. The F(ab’)2 fragments are highly useful for research interests, being able to penetrate tissue better than standard IgG antibodies and facilitate better antigen recognition while avoiding unspecific binding to Fc receptors.
Immobilized Pepsin Agarose Resin is an efficient method for producing F(ab’)2 fragments from IgG molecules for antibody mimetic. Undigested IgG can be quickly removed with our Protein A Agarose Resin (GoldBio Catalog # P-400). Fc fragments can also be removed using a gel filtration system.
Resin: 6% Cross-linked Agarose
Shipping/Handling: Shipped at ambient temperature. Upon receipt, store at 4°C, do NOT freeze.
Product Specifications
Catalog ID | I-030 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |