Phenylmethylsulfonyl fluoride (PMSF)

PMSF is a serine protease inhibitor that irreversibly inhibits serine proteases by sulfonylation of the serine residue in the active site of the protease. It is widely used in protocols for the isolation of proteins.

PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C. The half-life of a 20mM aqueous solution of PMSF is ~35 minutes at pH 8.0. This short half-life means that aqueous solutions of PMSF can be safely discarded after they have been rendered alkaline (pH >8.6) and stored for several hours at room temperature.

Product Specifications:
Phenylmethylsulfonyl fluoride; PMSF
Phenylmethanesulfonyl fluoride;
α-Toluenesulfonyl fluoride

MOLECULAR BIOLOGY GRADE

Formula: C₇H₇FO₂S

MW: 174.19 g/mol

White to slightly off white cyrstalline powder

Storage/Handling: Store desiccated at 4°C.
Soluble in DMSO or 100% Ethanol.
Protect from light. Hygroscopic.

PubChem Chemical ID: 4784

Note: To enable us to avoid hazardous shipping fees, U.S. domestic ground shipments of PMSF will be shipped as a limited quantity exception for the 25 g, 50 g, and 100 g pack sizes. In some instances GoldBio may ship 50 g as 2 x 25 g or 100 g as 4 x 25 g to qualify for a small quantity exception.