Hot Start Pfu DNA Polymerase
Hot Start Pfu DNA Polymerase is formulated with chemical modification which effectively neutralize 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities at room temperature, but regain the full enzyme activity upon the initial denaturation step. Hot start Pfu DNA polymerase retains the high fidelity, sensitivity and processivity of Pfu DNA polymerase, while provides reduced background by facilitating room temperature PCR assembly and preventing priming until stringent primer annealing temperatures are reached. Pfu DNA polymerase has an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.
- Hot Start Pfu DNA Polymerase
- 10x PCR Buffer with Mg2+
- 5x GC Enhancer
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 5 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.
Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.