Tris (Tris Base)
GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
A Tris buffer solution can be made by mixing Tris with Tris-HCl. This prevents overshooting the pH and prevents the need to work with strong acids or bases.
Tris, Tris Buffer
ULTRA PURE GRADEStorage/Handling: Store desiccated at room temperature.
PubChem CID: 6503
|Grade||ULTRA PURE GRADE|
|Storage/Handling||Store desiccated at room temperature.|
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This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.
Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.
A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.
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