Blue-White Screening
For today’s blog post, we’re going to shine a spotlight on a widely used technique in the field of...
X-Gal
Product Description
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5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.
X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Bacteria cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.
Many other applications also use X-Gal as a substrate to detect β-galactosidase activity. These include β-galactosidase -antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction and the detection of micrometastasis formation during tumor progession.
X-gal is commonly used in conjunction with IPTG (GoldBio #I2481) for blue-white screening.
Product Specifications:
X-Gal;
5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside
MOLECULAR BIOLOGY GRADE
Formula: C14H15BrClNO6
MW: 408.63 g/mol
Purity: Greater than 99% (HPLC)
Solution: Clear (2% in DMF)
Biological Activity: Blue color with Lambda, pUC, and M13
Storage/Handling: Store at -20°C. Protect from Light.
PubChem Chemical ID: 619018
Product Specifications
| Catalog ID | X4281 |
|---|---|
| CAS # | 7240-90-6 |
| MW | 408.63 g/mol |
| Grade | MOLECULAR BIOLOGY GRADE |
| Storage/Handling | Store at -20°C. Protect from light. |
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
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