Our most popular products from our most popular categories, from our Proven and Published™ Luciferin to our popular antibiotic lines and electrophoresis tools. GoldBio’s commitment to quality and affordability makes these products our top sellers.
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Enzyme activity can be slowed or stopped using inhibitors, like Proteinase K or RNase, while substrates like X-Gluc and X-Gal are used in detection and monitoring. D-Luciferin Potassium Salt, our most popular bioluminescent substrate, can be used for in vivo and in vitro imaging. GoldBio knows that having high quality enzyme tools are important to your laboratory, and we are committed to delivering quality products at a fraction of the price.
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GoldBio’s broad range of cell selection tools, including hundreds of antibiotics, antifungals and anticancers, plant selection and screening agents allow you to select, control and maintain your cell cultures with confidence and ease, no matter what cells you may be studying.
Antibiotics and Selection tools allow you to selectively control which cells can be found in your cultures.
GoldBio’s broad range of cell selection tools, including hundreds of antibiotics, antifungals and anticancers, plant selection and screening agents allow you to select, control and maintain your cell cultures with confidence and ease, no matter what cells you may be studying.
X-Gal
Product Description
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.
X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Bacteria cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.
Many other applications also use X-Gal as a substrate to detect β-galactosidase activity. These include β-galactosidase -antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction and the detection of micrometastasis formation during tumor progession.
X-gal is commonly used in conjunction with IPTG (GoldBio #I2481) for blue-white screening.
Product Specifications:
X-Gal;
5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside
MOLECULAR BIOLOGY GRADE
Formula: C14H15BrClNO6
MW: 408.63 g/mol
Purity: Greater than 99% (HPLC)
Solution: Clear (2% in DMF)
Biological Activity: Blue color with Lambda, pUC, and M13
Storage/Handling: Store at -20°C. Protect from Light.
PubChem Chemical ID:
619018
X-Gal Quick Answers
How do you make X-Gal stock solution?
How much X-Gal do you use for blue-white screening?
What is X-Gal?
X-gal is the chromogenic substrate for the enzyme β-galactosidase. When X-gal is cleaved by
β-galactosidase, it forms galactose and 5-bromo-4-chloro-3-hydroxyindole.
5-bromo-4-chloro-3-hydroxyindole can dimerize and then is oxidized, forming 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product.
The blue product suggests the
presence of β-galactosidase. Therefore, this substrate is
often used as a reporter in molecular biology.
How do you make X-Gal stock solution?
Instructions to prepare a 20 mg/ml X-gal stock solution are as follows
1. Weigh 200 mg of X-Gal into a 15 ml polypropylene centrifuge tube.
2. Add 10 mL of Dimethylformamide (DMF). Vortex to dissolve the X-Gal.
3. Store at -20°C. Wrap the centrifuge tube in aluminum foil to protect from light.
Note: The X-Gal solution is stable for 6-12 months unless improperly handled. We recommend making 1 ml aliquot portions of the solution to minimize degradation if there will be frequent small scale use of the X-Gal solution.
How much X-Gal do you use for blue-white screening?
For blue-white screening, use 10 µl per 1 mL of media if you’re using a 20 mg/ml stock solution. If you’re using a 100 mg/ml stock solution, which is only stable for 1 week, use 2 µl.
How do you store X-Gal?
- Store powder X-gal at -20°C. Protect from Light.
- Store X-gal stock solution at -20°C. It’s important to protect your stored stock solution from light. You can do this by wrapping the tube in aluminum foil.
Product Specifications
Catalog ID | X4281 |
---|---|
CAS # | 7240-90-6 |
MW | 408.63 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at -20°C. Protect from light. |