Hot Start Taq DNA Polymerase plus dNTP
Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´ flap endonuclease activity. Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.
- Hot Start Taq DNA Polymerase
- 10x PCR Buffer with Mg2+
- 5x GC Enhancer
- 10mM dNTP mix
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 5 units/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.
Source: E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.