Taq DNA Polymerase with Dye
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Taq polymerase is ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg 2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%. The 10x buffer also includes an inert blue dye for visible tracking and the prevention of pipetting mistakes, wasted time, reagents and money.
- Taq DNA Polymerase
- 10x PCR Buffer with Mg2+ with convenience dye
- 5x GC Enhancer
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 1 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.
Source: E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.