Proteinase K Solution 20 mg/ml
GoldBio's Proteinase K solution is pre-made in 20 mg/ml concentration aliquots so that you don't have to worry about making it yourself. Now you can have the quality products that you have come to know and count on from GoldBio and combine it with the convenience of a pre-made solution.
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.
Proteinase K is typically used at a concentration of 50-100 μg/ml. It is active with or without the presence of SDS, urea, EDTA or various metal ions, but the activity of proteinase K can be increased by adding the denaturing agents and the structure of proteinase K can be stabilized by addition of Ca 2+. Proteinase K is inactivated by heating to 95°C for 10 minutes or using an inhibitor such as PMSF, AEBSF or DFP.
Proteinase K Solution - 20 mg/ml
MOLECULAR BIOLOGY GRADE
MW: 28.5 kDa
Activity: ≥30 U/mg
Unit Definition: One unit is defined as the amount of enzyme that will liberate 1.0 μmol tyrosine (Folin-positive amino acid) from casein per minute at 37°C, pH 7.5.
Storage/Handling: Store at -20°C.
Proteinase K Solution is provided in 50mM Tris-HCl (pH 8.0), 3mM CaCl2 and 50% glycerol.
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A general protocol for inactivating nucleases with Proteinase K during the extraction of nucleic acids
A protocol for the extraction of DNA from mouse tail using Proteinase K
Your free, printable guide to proteinase K, with general info, usage details and how to activate and optimize.
GoldBio Proteinase K efficacy and analyses; including DNase/RNase contamination assays and DNA extraction assays.
The Proteinase K Digestion Guide includes 4 tables showing the experimental setup for proteinase K digestion and includes the referenced publication.