certus QC - mycoplasma Detection Kit

Product Description

Certus QC - mycoplasma detection kit is a ready-to-use isothermal amplification based detection assay for simple, rapid and highly sensitive detection of Mycoplasma and Acholeplasma species in cell culture.

Don’t risk the quality and safety of your important cell culture experiments, primary and continuous cell lines to bacterial contamination. In less than one hour, the certus QC – mycoplasma detection kit provides you with highly sensitive results, detecting the most common Mycoplasma species that account for >95% of contamination (see species list below).

Save Time and Money

Traditional microbiological detection of Mycoplasma species requires a testing period of 28 days, bogging down workflows, delaying projects and putting a hold on publication. Instead, get real-time data that allows you to move forward with important project decisions.

Additionally, other PCR-based and qPCR-based detection methods can take several hours and require an extraction step, which either requires a kit add-on or additional reagents and time. The certus QC – mycoplasma detection kits and certus QC - mycoADVANCED detection kits do not require a DNA extraction step, which saves you considerable time and money.

Rapid, superior mycoplasma detection kits


Get the Most Reliable Results

Isothermal amplification detection delivers the most accurate results. There is no worry over false negatives, which can occur through bioluminescence detection. More sensitivity allows you to not only move forward with confidence, but to publish with confidence.


Benefits

  • No DNA extraction required. Save money and time with this all-in-one kit.
  • Reproducible and reliable results in less than 1 hour.
  • Highly sensitive
  • Works in most instruments.
  • Minimal hands-on time
  • Extremely user-friendly.
  • Cost-effective
  • A positive assay control, which consists of an artificial DNA template containing the Mycoplasma target sequence. It is not infectious and cannot cause contamination in cell lines.
  • A negative assay control, which consists of your own medium diluted in lysis buffer.

Kit Components

Certus QC – mycoplasma detection kits components are color-coded for ease of use.

  • Primer Mix (Includes primers for an internal control (IC), which is only amplified in Mycoplasma-negative or low positive samples.)
  • Enzyme Mix
  • A positive assay control, which consists of an artificial DNA template containing the Mycoplasma target sequence. It is not infectious and cannot cause contamination in cell lines.
  • Lysis Buffer (A negative assay control can be made from diluting fresh medium in lysis buffer.) Also includes an internal control (IC), which is only amplified in Mycoplasma-negative or low positive samples.

Species Detection

The following common Acholeplasma and Mycoplasma species have been tested and detected:

  • A. laidlawii
  • M. arginini
  • M. fermentans
  • M. hominis
  • M. hyorhinis
  • M. orale
  • M. synoviae

Additional Mycoplasma species that have been tested and detected include:

  • M. adleri
  • M. arthritidis
  • M. bovirhinis
  • M. bovis
  • M. bovoculi
  • M. californicum
  • M. ciconiae
  • M. cottewii
  • M. dispar
  • M. fastidiosum
  • M. feriruminatoris
  • M. gallinarum
  • M. genitalium
  • M. lagogenitalium
  • M. leachii
  • M. meleagridis
  • M. microti
  • M. mucosicanis
  • M. neophronis
  • M. penetrans
  • M. pulmonis
  • M. salivarium
  • M. sturnidae
  • M. sualvi

Storage/Handling:

Components of the kit may be shipped on blue ice and remain stable during transit. Upon arrival, store kit at -20°C. See vial label for expiration date. Repeated freeze-thaw cycles may impact the quality of the primers. Aliquot the Primer Mix (vial #1) prior to storage.

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