i7® Hot Start High-Fidelity DNA Polymerase

Product Description

i7® Hot Start High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. i7 Hot Start High-Fidelity DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. i7® Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long (up to 20 kb) or difficult amplicons. This product is supplied with a proprietary 2.5X PCR reaction buffer containing MgCl2 with a final (1X) concentration of 2mM. This buffer allows for amplification of non GC rich templates as well as GC rich templates up to 84%.

Applications

  • Long and difficult template DNA amplification
  • Cloning
  • High-fidelity PCR

Product Includes

  • i7® Hot Start High-Fidelity DNA Polymerase
  • 2.5X - i7 PCR Buffer with Mg2+

Activity data
i7® Hot Start High-Fidelity DNA Polymerase generates robust and high-quality PCR products with difficult templates (Fig. A). PCR extension temperatures can be used between 68 to 72º C (Fig. B). This enzyme is resistant to different PCR inhibitors such as heparin, humic acid and xylan (Fig. C).

Hot Start High Fidelity DNA Polymerase I7 image PCR difficult templateHot Start High Fidelity DNA Polymerase I7 PCR Extension Temperatures

Hot Start High Fidelity DNA Polymerase I7 PCR Inhibitor

Storage Buffer:

50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, pH 7.5 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72°C.

Heat Inactivation: No

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Technical Documentation

P Protocol
O Other
C Certificate of Analysis
S Safety Data Sheet

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