i7® Hot Start High-Fidelity DNA Polymerase
i7® Hot Start High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. i7 Hot Start High-Fidelity DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. i7® Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long (up to 20 kb) or difficult amplicons. This product is supplied with a proprietary 2.5X PCR reaction buffer containing MgCl2 with a final (1X) concentration of 2mM. This buffer allows for amplification of non GC rich templates as well as GC rich templates up to 84%.
- Long and difficult template DNA amplification
- High-fidelity PCR
- i7® Hot Start High-Fidelity DNA Polymerase
- 2.5X - i7 PCR Buffer with Mg2+
i7® Hot Start High-Fidelity DNA Polymerase generates robust and high-quality PCR products with difficult templates (Fig. A). PCR extension temperatures can be used between 68 to 72º C (Fig. B). This enzyme is resistant to different PCR inhibitors such as heparin, humic acid and xylan (Fig. C).
50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, pH 7.5 @ 25°C
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72°C.
Heat Inactivation: No
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.