This is the hub page for for CRISPR, PCR and DNA Gel Electrophoresis.
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Find out more about how our reliable enzymes, products, protocols, troubleshooting tips and resources can help you study and manipulate nucleic acids and genetic material. We offer high-quality PCR reagents for your DNA amplification reaction: dNTPs, PCR additives, and Taq, High Fidelity and Hot Start DNA Polymerases. Also, our InstantAmp™ Plant Direct PCR Kit is designed to be the simplest and best method to efficiently amplify DNA fragments from plant tissues. In addition, we provide a wide range of products including reverse transcriptase (RT) and reverse transcription-polymerase chain reaction (RT-PCR) Kits that allow cDNA generation and detection of RNA. We also carry efficient ligases, T7 exonuclease and T7 endonuclease I that are ideal for nucleic acid manipulation.
Using quantitative PCR (qPCR), also known as real time quantitative PCR (RT-qPCR) and quantitative real time PCR (qRT-PCR), we can analyze the quantity (copy number) of a target DNA sequence as it is replicated, in real time. Here, you can find GoldBio products ideal for robust amplification and accurate gene expression quantification. Our EvaGreen® dye, a great alternative to SYBR® Green I, Goof-Proof™ qPCR Master Mix, and RT-qPCR Kit are optimal for use in your DNA melt curve analysis and real time-PCR (RT-PCR) reactions.
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This molecular copying operates through cycles of thermal reactions enabled by an assembly of biochemical reagents, and amplifies a few copies of DNA to millions. Amplified DNA can then be analyzed qualitatively (for its presence), quantitatively (by amount) or sequentially (for its genetic code), and used in downstream molecular biology applications. 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CRISPR Products & Resources
The CRISPR/Cas gene editing system is a breakthrough technology currently being used in many labs to modify genomes. Learn about or trusted CRISPR products and supporting tools here. We offer Cas9 nucleases, a T7 endonuclease, Pfu DNA Polymerases as well as comprehensive protocols to perform CRISPR-mediated genome manipulation. We also offer informative articles discussing the uses–transcriptional regulation, epigenetic modification, nucleic acid modification, RNA editing and ssDNA detection–and downstream applications of CRISPR.
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DNA Gel Electrophoresis Products & Resources
Gel electrophoresis is a technique commonly used to separate DNA fragments by size. We have developed a collection of high purity agarose products–Agarose LE, Low Melt Agarose and High Resolution Agarose–to help you generate an efficient agarose gel for accurate separation of your nucleic acid samples. In addition, you can find a variety of DNA ladders and loading dyes here, including our ultra-sensitive fluorescent nucleic acid dye 6x GelRed™, which is a safer alternative to ethidium bromide (EtBr).
Our ladder comparison charts, comprehensive protocols and video demonstrations also found below will guide you through the DNA gel electrophoresis process.
PCR Cloning Kit
Product Description
Our PCR Cloning kit utilizes cloning technology that is simple, rapid and highly efficient which allows direct cloning of any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by a high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 minutes) with more than 95% cloning efficiency.
Applications
- Clone any insert at any site within any vector
- Restriction enzyme and phosphatase free system
- Joining multiple large fragments at once
- Precise insertion at a desired orientation
- Rapid and high efficiency with >95% positive clones
Product Includes
- 5X enzyme premix
- 2X PCR premix
- High efficiency competent cells (DH10B chemically competent cells)
Storage/Handling: This product may be shipped on dry ice. Competent cells should be stored at -80°C, enzyme and PCR premix should be stored at -20°C. Product should be stored immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
