Highest Density Nickel
GoldBio’s new Highest Density Nickel Agarose is a high capacity resin manufactured with a polychelator ligand. The product was developed to work in the presence of EDTA and DTT without any loss of performance. Its specificity and stability allows a one-step purification, eliminating the need for pretreatment of samples that cause nickel stripping.
Our Highest Density Nickel Agarose is supplied as a suspension in 50% aqueous suspension containing 20% ethanol. Purifications can be performed using 20mM EDTA and 20mM DTT and can work in both native as well as denaturing conditions.
Bead Geometry/Size: Spherical - ~50-150 µm
Crosslinking: Highly Crosslinked
- 20mM DTT
- 20mM EDTA
- 8M urea
- 6M guanidine hydrochloride
- 30% acetonitrile
- 100% methanol
- 100% ethanol
- Buffer solutions at pH 4 - 9
Loading Capacity: >60 μmol me2+/ml gel
Static Binding Capacity: ≥80 mg/ml gel
Antimicrobial Agent: 20% ethanol
Storage/Handling: Store at 4°C. Do NOT freeze.
Detailed Instructions for Agarose Bead Use, Column Packing and Regeneration
Quick and Simple His-Tag Column Prep protocol including Pouring and Packing an Agarose Column