Taq DNA Polymerase 2x Premix with Dye

Product Description

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Taq polymerase is ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg 2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

The 2x Premix with Dye comes ready to use, containing Taq DNA Polymerase, dNTPs, MgCl2, stabilizers and includes an inert blue dye for visible tracking and the prevention of pipetting mistakes, wasted time, reagents and money. It has been optimized for routine PCR applications.

Product Includes

  • Taq DNA Polymerase 2x Premix with Dye
  • 5x GC Enhancer

Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Taq Concentration (1x): 0.05 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

Source: E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product Specifications

Catalog ID T-518
Storage/Handling Store at -20°C.

Technical Documentation

P Protocol
O Other
C Certificate of Analysis
S Safety Data Sheet

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