• object(anvilstl\commercio\models\Category)#394 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Dna-Protien-Electrophoresis.jpg" ["medium"]=> string(97) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Dna-Protien-Electrophoresis.jpg" ["large"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Dna-Protien-Electrophoresis.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Dna-Protien-Electrophoresis.jpg" ["medium"]=> string(97) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Dna-Protien-Electrophoresis.jpg" ["large"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Dna-Protien-Electrophoresis.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(198) ["root"]=> int(393) ["lft"]=> int(40) ["rgt"]=> int(45) ["level"]=> int(1) ["name"]=> string(35) "DNA & Protein Electrophoresis (old)" ["url_name"]=> string(27) "dna-protein-electrophoresis" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(439) "

    Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.

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    Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.

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    Gold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.

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    Gold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.

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  • object(anvilstl\commercio\models\Category)#425 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(353) ["root"]=> int(1) ["lft"]=> int(236) ["rgt"]=> int(237) ["level"]=> int(3) ["name"]=> string(12) "PCR Products" ["url_name"]=> string(12) "pcr-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(755) "PCR (Polymerase Chain Reaction) is one of the most valuable, if not THE most valuable, biochemical techniques used in every molecular biology and biochemistry lab. It is the hallmark of our scientific molecular era, utilized for copying, sequencing or quantifying DNA or RNA. PCR is the process of using thermocycling with heat resistant enzymes to quickly and reliably copy strands of DNA. High quality PCR results save hours of needless replication and frustration and high quality ingredients are essential for producing those results. GoldBio offers the best quality ingredients for your PCR in order to get the most reliable, crisp and clear bands on your gel for use in downstream cloning or if you just need to quickly sequence the PCR strand." 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PCR is the process of using thermocycling with heat resistant enzymes to quickly and reliably copy strands of DNA. High quality PCR results save hours of needless replication and frustration and high quality ingredients are essential for producing those results. GoldBio offers the best quality ingredients for your PCR in order to get the most reliable, crisp and clear bands on your gel for use in downstream cloning or if you just need to quickly sequence the PCR strand." 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  • object(anvilstl\commercio\models\Category)#427 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(91) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/ABX Petri with letters.jpg" ["medium"]=> string(92) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/ABX Petri with letters.jpg" ["large"]=> string(91) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/ABX Petri with letters.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(91) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/ABX Petri with letters.jpg" ["medium"]=> string(92) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/ABX Petri with letters.jpg" ["large"]=> string(91) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/ABX Petri with letters.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(375) ["root"]=> int(1) ["lft"]=> int(204) ["rgt"]=> int(205) ["level"]=> int(3) ["name"]=> string(15) "Cloning Enzymes" ["url_name"]=> string(15) "cloning-enzymes" ["title"]=> string(0) "" ["short_description"]=> string(135) "

    Page that is linked to DNA amplification page (generated precisely for DNA amplification hub page). Houses our cloning enzymes.

    " ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(38) ["banner_image_id"]=> int(0) ["meta_description"]=> string(143) "GoldBio offers cloning enzymes including Hot Start Pfu DNA polymerases, ligases, T4 Polynucleotide Kinase to help you achieve cloning success." ["meta_keywords"]=> string(169) "cloning, DNA, enzymes, ligase, ligation, protocol, method, molecular cloning, T4 enzyme, DNA polymerase, blue white screening of colonies, antibiotics, ligation protocol" ["root_sort"]=> int(1551193699) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(3) ["items_per_page"]=> int(25) ["color"]=> string(0) "" } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(375) ["root"]=> int(1) ["lft"]=> int(204) ["rgt"]=> int(205) ["level"]=> int(3) ["name"]=> string(15) "Cloning Enzymes" ["url_name"]=> string(15) "cloning-enzymes" ["title"]=> string(0) "" ["short_description"]=> string(135) "

    Page that is linked to DNA amplification page (generated precisely for DNA amplification hub page). Houses our cloning enzymes.

    " ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(38) ["banner_image_id"]=> int(0) ["meta_description"]=> string(143) "GoldBio offers cloning enzymes including Hot Start Pfu DNA polymerases, ligases, T4 Polynucleotide Kinase to help you achieve cloning success." 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array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(2) { [0]=> array(7) { ["id"]=> int(6) ["name"]=> string(13) "Content Block" ["slug"]=> string(13) "content-block" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(2) { [0]=> array(21) { ["id"]=> int(41) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [1]=> array(22) { ["id"]=> int(42) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(7) "Content" ["slug"]=> string(7) "content" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "RichTextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(482) "

    In molecular cloning, recombined DNA is generated and inserted into a host for replication. This process requires reliable and efficient enzymes as well as high quality reagents. GoldBio offers multiple cloning enzymes including our Hot Start Pfu DNA polymerases, T4 DNA ligases, T4 DNA polymerases, T4 Polynucleotide Kinase (PNK) to help you achieve cloning success. Here you will also find detailed and comprehensive protocols for different stages of the cloning process.

    " } } } [1]=> array(7) { ["id"]=> int(1) ["name"]=> string(6) "Banner" ["slug"]=> string(6) "banner" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(4) { [0]=> array(21) { ["id"]=> int(28) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [1]=> array(21) { ["id"]=> int(29) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> 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string(0) "" ["type"]=> string(10) "ImageField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(4) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["large"]=> string(117) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/banner_image/large/DNA Amplification - Blog Banner.png" ["medium"]=> string(118) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/banner_image/medium/DNA Amplification - Blog Banner.png" ["original"]=> string(120) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/banner_image/original/DNA Amplification - Blog Banner.png" ["small"]=> string(117) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/banner_image/small/DNA Amplification - Blog Banner.png" } } } } } } [1]=> array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(1) { [0]=> array(7) { ["id"]=> int(17) ["name"]=> string(16) "Resource Section" ["slug"]=> string(16) "resource-section" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(2) { [0]=> array(22) { ["id"]=> int(67) ["parent_id"]=> int(0) ["field_group_id"]=> int(17) ["name"]=> string(13) "Section Ttile" ["slug"]=> string(13) "section-title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(17) "Cloning Resources" } [1]=> array(22) { ["id"]=> int(68) ["parent_id"]=> int(0) ["field_group_id"]=> 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["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(1) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(17) "Ligation Protocol" } [1]=> array(21) { ["id"]=> int(50) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "TextAreaField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [2]=> array(22) { ["id"]=> int(51) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(3) "Url" ["slug"]=> string(3) "url" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(8) "UrlField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(3) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(90) "https://www.goldbio.com/documents/3886/Ligation%20Protocol%20using%20T4%20DNA%20Ligase.pdf" } [3]=> array(22) { ["id"]=> int(52) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(10) "Icon Image" ["slug"]=> string(5) "image" ["placeholder"]=> NULL ["instructions"]=> string(42) "Use square images (75x75) for best results" ["type"]=> string(10) "ImageField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(4) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["small"]=> string(95) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/small/Protocols - NW.png" ["medium"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/medium/Protocols - NW.png" ["large"]=> string(95) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/Protocols - NW.png" ["original"]=> string(98) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/Protocols - NW.png" } } } } [1]=> array(7) { ["id"]=> int(8) ["name"]=> string(13) "Resource Card" ["slug"]=> string(13) "resource-card" ["description"]=> NULL ["show_in_picker"]=> int(0) ["deleted_on"]=> NULL ["fields"]=> array(4) { [0]=> array(22) { ["id"]=> int(49) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(1) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(21) "Blue-White Screening " } [1]=> array(21) { ["id"]=> int(50) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "TextAreaField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> 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"https://www.goldbio.com/articles/article/a-guide-for-choosing-between-commonly-used-antibiotics" } [3]=> array(22) { ["id"]=> int(52) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(10) "Icon Image" ["slug"]=> string(5) "image" ["placeholder"]=> NULL ["instructions"]=> string(42) "Use square images (75x75) for best results" ["type"]=> string(10) "ImageField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(4) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["small"]=> string(93) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/small/GoldBio-6142.jpg" ["medium"]=> string(94) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/medium/GoldBio-6142.jpg" ["large"]=> string(93) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/GoldBio-6142.jpg" ["original"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/GoldBio-6142.jpg" } } } } } } } } } } } }

    • Pfu 2x DNA Polymerase Master Mix

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    Product Description

    Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

    The 2x Premix with Dye comes ready to use, containing Pfu DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications.

    Product Includes

    • Pfu DNA Polymerase 2x Premix
    • 5x GC Enhancer

    Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

    Pfu Concentration (1x): 0.1 unit/μl
    One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

    Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus

    Storage/Handling: Store at -20°C.
    Product may be shipped on blue ice.

    Product Specifications

    Catalog ID P-660

    Technical Documentation

    P Protocol
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    C Certificate of Analysis
    S Safety Data Sheet
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    PCR Protocol using Pfu DNA Polymerase 2X Master Mix

    PCR utilizing Pfu DNA polymerase 2X master mix.

    S
    SDS (MSDS) - Pfu 2x DNA Polymerase Master Mix

    Safety Data Sheet

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