Pfu 2x DNA Polymerase Master Mix
Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.
The 2x Premix with Dye comes ready to use, containing Pfu DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications.
- Pfu DNA Polymerase 2x Premix
- 5x GC Enhancer
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Pfu Concentration (1x): 0.1 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.
Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.