Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.
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plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.
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that is linked to DNA amplification page (generated precisely for DNA amplification hub page). Houses our cloning enzymes.
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This process requires reliable and efficient enzymes as well as high quality reagents. GoldBio offers multiple cloning enzymes including our Hot Start Pfu DNA polymerases, T4 DNA ligases, T4 DNA polymerases, T4 Polynucleotide Kinase (PNK) to help you achieve cloning success. Here you will also find detailed and comprehensive protocols for different stages of the cloning process.
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"https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/GoldBio-6142.jpg" ["original"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/GoldBio-6142.jpg" } } } } } } } } } } } }Pfu 2x DNA Polymerase Master Mix
Product Description
Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.
The 2x Premix with Dye comes ready to use, containing Pfu DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications.
Product Includes
- Pfu DNA Polymerase 2x Premix
- 5x GC Enhancer
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Pfu Concentration (1x): 0.1 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.
Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.
Product Specifications
Catalog ID | P-660 |
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