Pfu DNA Polymerase plus dNTP
Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.
- Pfu DNA Polymerase
- 10x PCR Buffer with Mg2+
- 5x GC Enhancer
- 10mM dNTP mix
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 5 unit/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.
Source: E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.