Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.
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cellular functions and are vital for effective research. Enzyme activity can be slowed or stopped using inhibitors, like Proteinase K or RNase, while substrates like X-Gluc and X-Gal are used in detection and monitoring. D-Luciferin Potassium Salt, our most popular bioluminescent substrate, can be used for in vivo and in vitro imaging. GoldBio knows that having high quality enzyme tools are important to your laboratory, and we are committed to delivering quality products at a fraction of the price.
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Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.
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that is linked to DNA amplification page (generated precisely for DNA amplification hub page). Houses our cloning enzymes.
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[3]=> array(7) { ["id"]=> int(8) ["name"]=> string(13) "Resource Card" ["slug"]=> string(13) "resource-card" ["description"]=> NULL ["show_in_picker"]=> int(0) ["deleted_on"]=> NULL ["fields"]=> array(4) { [0]=> array(22) { ["id"]=> int(49) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(1) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(55) "A Guide for Choosing Between Commonly Used Antibiotics" } [1]=> array(21) { ["id"]=> int(50) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "TextAreaField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [2]=> array(22) { ["id"]=> int(51) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(3) "Url" ["slug"]=> string(3) "url" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(8) "UrlField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(3) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(95) "https://www.goldbio.com/articles/article/a-guide-for-choosing-between-commonly-used-antibiotics" } [3]=> array(22) { ["id"]=> int(52) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(10) "Icon Image" ["slug"]=> string(5) "image" ["placeholder"]=> NULL ["instructions"]=> string(42) "Use square images (75x75) for best results" ["type"]=> string(10) "ImageField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(4) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["small"]=> string(93) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/small/GoldBio-6142.jpg" ["medium"]=> string(94) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/medium/GoldBio-6142.jpg" ["large"]=> string(93) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/GoldBio-6142.jpg" ["original"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/GoldBio-6142.jpg" } } } } } } } } } } } }T4 Polynucleotide Kinase (PNK)
Product Description
T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5’-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3’-monophosphates. In the presence of ADP, T4 PNK exhibits 5’-phosphatase activity and catalyzes the exchange of phosphate group between 5’-P-oligonucleotides/polynucleotides and ATP. The enzyme is also a 3’-phosphatase.
NOTE: T4 PNK requires ATP for activity, but it is not supplied with ATP because it interferes with radiolabeling reactions.
Product Includes
- T4 Polynucleotide Kinase (PNK)
- 10x T4 PNK Reaction Buffer
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 10 unit/μl
One unit of T4 Polynucleotide Kinase converts 1 nmol of 32P from [γ-32P]-ATP into an acid-insoluble form in 30 minutes at 37 °C under standard assay conditions.
Source: E. coli cells with a cloned pseT gene of bacteriophage T4
Inhibition and Inactivation:
Inhibitors: metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50mM.
Inactivated by heating at 70°C for 15 minutes or by addition of EDTA.
Quality Control
T4 PNK is tested in 5´ phosphorylation of nucleic acids and is free from exo- and endonuclease and RNase activities.
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.
Product Specifications
Catalog ID | T-413 |
---|---|
Storage/Handling | Store at -20°C. |