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Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.

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Gold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.

" ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(27) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> string(22) "rgba(130, 254, 226, 1)" } ["_related":"yii\db\BaseActiveRecord":private]=> array(0) { } ["_relationsDependencies":"yii\db\BaseActiveRecord":private]=> array(0) { } ["_errors":"yii\base\Model":private]=> NULL ["_validators":"yii\base\Model":private]=> NULL ["_scenario":"yii\base\Model":private]=> string(7) "default" ["_events":"yii\base\Component":private]=> array(6) { ["beforeInsert"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#426 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeInsert" } [1]=> NULL } } ["afterInsert"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#426 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" 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["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeDelete" } [1]=> NULL } } ["afterDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#426 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) 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object(anvilstl\commercio\models\Category)#427 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(226) ["root"]=> int(1) ["lft"]=> int(19) ["rgt"]=> int(20) ["level"]=> int(2) ["name"]=> string(10) "K_Products" ["url_name"]=> string(10) "k-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(226) ["root"]=> int(1) ["lft"]=> int(19) ["rgt"]=> 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["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }

object(anvilstl\commercio\models\Category)#429 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(231) ["root"]=> int(1) ["lft"]=> int(29) ["rgt"]=> int(30) ["level"]=> int(2) ["name"]=> string(10) "P_Products" ["url_name"]=> string(10) "p-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(231) ["root"]=> int(1) ["lft"]=> int(29) ["rgt"]=> 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object(anvilstl\commercio\models\Category)#431 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(234) ["root"]=> int(1) ["lft"]=> int(37) ["rgt"]=> int(38) ["level"]=> int(2) ["name"]=> string(10) "T_Products" ["url_name"]=> string(10) "t-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(234) ["root"]=> int(1) ["lft"]=> int(37) ["rgt"]=> 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["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }

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Page that is linked to DNA amplification page (generated precisely for DNA amplification hub page). Houses our cloning enzymes.

" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(38) ["banner_image_id"]=> int(0) ["meta_description"]=> string(143) "GoldBio offers cloning enzymes including Hot Start Pfu DNA polymerases, ligases, T4 Polynucleotide Kinase to help you achieve cloning success." 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array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(2) { [0]=> array(7) { ["id"]=> int(6) ["name"]=> string(13) "Content Block" ["slug"]=> string(13) "content-block" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(2) { [0]=> array(21) { ["id"]=> int(41) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [1]=> array(22) { ["id"]=> int(42) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(7) "Content" 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In molecular cloning, recombined DNA is generated and inserted into a host for replication. This process requires reliable and efficient enzymes as well as high quality reagents. GoldBio offers multiple cloning enzymes including our Hot Start Pfu DNA polymerases, T4 DNA ligases, T4 DNA polymerases, T4 Polynucleotide Kinase (PNK) to help you achieve cloning success. Here you will also find detailed and comprehensive protocols for different stages of the cloning process.

" } } } [1]=> array(7) { ["id"]=> int(1) ["name"]=> string(6) "Banner" ["slug"]=> string(6) "banner" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(4) { [0]=> array(21) { ["id"]=> int(28) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [1]=> array(21) { ["id"]=> int(29) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL 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T4 Polynucleotide Kinase (PNK)

Name: T4 Polynucleotide Kinase (PNK)
SKU: T-413
Availability: InStock

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Product Description

T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5’-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3’-monophosphates. In the presence of ADP, T4 PNK exhibits 5’-phosphatase activity and catalyzes the exchange of phosphate group between 5’-P-oligonucleotides/polynucleotides and ATP. The enzyme is also a 3’-phosphatase.

NOTE: T4 PNK requires ATP for activity, but it is not supplied with ATP because it interferes with radiolabeling reactions.

Product Includes

T4 Polynucleotide Kinase (PNK)
10x T4 PNK Reaction Buffer

Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Concentration: 10 unit/μl
One unit of T4 Polynucleotide Kinase converts 1 nmol of 32P from [γ-32P]-ATP into an acid-insoluble form in 30 minutes at 37 °C under standard assay conditions.

Source: E. coli cells with a cloned pseT gene of bacteriophage T4

Inhibition and Inactivation:
Inhibitors: metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50mM.
Inactivated by heating at 70°C for 15 minutes or by addition of EDTA.

Quality Control
T4 PNK is tested in 5´ phosphorylation of nucleic acids and is free from exo- and endonuclease and RNase activities.

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Product Specifications

Catalog ID	T-413
Storage/Handling	Store at -20°C.

Technical Documentation

P Protocol

O Other

C Certificate of Analysis

S Safety Data Sheet

Phosphorylation Protocol using T4 PNK

Phosphorylation protocol utilizing T4 Polynucleotide Kinase.

SDS (MSDS) - T4 Polynucleotide Kinase (PNK)

Safety Data Sheet

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A-081-1	1 g	$ 28.00
A-081-5	5 g	$ 40.00
A-081-25	25 g	$ 106.00
A-081-100	100 g	$ 386.00
A-081-250	250 g	$ 763.00