T4 Polynucleotide Kinase (PNK)
T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5’-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3’-monophosphates. In the presence of ADP, T4 PNK exhibits 5’-phosphatase activity and catalyzes the exchange of phosphate group between 5’-P-oligonucleotides/polynucleotides and ATP. The enzyme is also a 3’-phosphatase.
NOTE: T4 PNK requires ATP for activity, but it is not supplied with ATP because it interferes with radiolabeling reactions.
- T4 Polynucleotide Kinase (PNK)
- 10x T4 PNK Reaction Buffer
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 10 unit/μl
One unit of T4 Polynucleotide Kinase converts 1 nmol of 32P from [γ-32P]-ATP into an acid-insoluble form in 30 minutes at 37 °C under standard assay conditions.
Source: E. coli cells with a cloned pseT gene of bacteriophage T4
Inhibition and Inactivation:
Inhibitors: metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50mM.
Inactivated by heating at 70°C for 15 minutes or by addition of EDTA.
T4 PNK is tested in 5´ phosphorylation of nucleic acids and is free from exo- and endonuclease and RNase activities.
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.
|Storage/Handling||Store at -20°C.|