An Introduction to Isothermal Amplification
What is Isothermal Amplification
In molecular biology, when it comes to copying nucleic acid sequenc...
T4 Polynucleotide Kinase (PNK)
Product Description
T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5’-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3’-monophosphates. In the presence of ADP, T4 PNK exhibits 5’-phosphatase activity and catalyzes the exchange of phosphate group between 5’-P-oligonucleotides/polynucleotides and ATP. The enzyme is also a 3’-phosphatase.
NOTE: T4 PNK requires ATP for activity, but it is not supplied with ATP because it interferes with radiolabeling reactions.
Product Includes
- T4 Polynucleotide Kinase (PNK)
- 10x T4 PNK Reaction Buffer
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 10 unit/μl
One unit of T4 Polynucleotide Kinase converts 1 nmol of 32P from [γ-32P]-ATP into an acid-insoluble form in 30 minutes at 37 °C under standard assay conditions.
Source: E. coli cells with a cloned pseT gene of bacteriophage T4
Inhibition and Inactivation:
Inhibitors: metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50mM.
Inactivated by heating at 70°C for 15 minutes or by addition of EDTA.
Quality Control
T4 PNK is tested in 5´ phosphorylation of nucleic acids and is free from exo- and endonuclease and RNase activities.
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.
Product Specifications
| Catalog ID | T-413 |
|---|---|
| Storage/Handling | Store at -20°C. |
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
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