T4 UvsX DNA Recombinase
T4 UvsX DNA Recombinase is a recombinase used in an isothermal amplification method where the recombinase complexes with the DNA primer. The recombinase-primer complex scans double-stranded DNA for a homologous sequence, which is followed by a strand exchange. During this process, the displaced strand is stabilized by bound T4 gp32 protein, and amplification can begin.
This isothermal reaction employing T4 UvsX DNA Recombinase does not require pretreatment of DNA, nor does it require any strand denaturation, making it suitable for field tests or performing tests with minimal equipment and supplies. Furthermore, isothermal amplification is an extremely sensitive technique.
T4 UvsX DNA Recombinase and other recombinases form presynaptic filaments on ssDNA that are activated to search for homology in dsDNA and to perform DNA strand exchange. UvsX recombinase is free from detectable nuclease activities. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. Recombinases of the RecA/RAD51 family perform the central catalytic role in the process of error-free repair of DNA double-strand breaks and for replication fork restart.
- UvsX Recombinase
- 10X UvsX Recombinase Reaction Buffer
Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 5 μg/μl
Source: E. coli BL21 (DE3) strain expressing T4 UvsX gene
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.
|Name||T4 UvsX DNA Recombinase|
|Storage/Handling||Store at -20°C.|