Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.
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plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.
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that is linked to DNA amplification page (generated precisely for DNA amplification hub page). Houses our cloning enzymes.
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This process requires reliable and efficient enzymes as well as high quality reagents. GoldBio offers multiple cloning enzymes including our Hot Start Pfu DNA polymerases, T4 DNA ligases, T4 DNA polymerases, T4 Polynucleotide Kinase (PNK) to help you achieve cloning success. Here you will also find detailed and comprehensive protocols for different stages of the cloning process.
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"https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/GoldBio-6142.jpg" ["original"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/GoldBio-6142.jpg" } } } } } } } } } } } }Taq DNA Ligase
Product Description
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in
duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini,
using NAD+ as a cofactor. The ligation will occur only if the
oligonucleotides are perfectly paired to the complementary target DNA
and have no gaps between them; therefore, a single-base substitution can
be detected.
Taq DNA Ligase is active at elevated temperatures
(45-70°C).
Product Includes
- Taq DNA Ligase
- 10X Taq DNA Ligase Buffer with NAD+
Purity: ≥99% (assessed by SDS-PAGE with Coomassie blue staining)
Concentration: 40 Units/μl
Unit Definitions: One unit is defined as the amount of Taq DNA Ligase required to join 50%
of 1 μg of the 12-base cohesive ends of Lambda DNA cut with Sma I and
Sal I in 50 μl reaction in 15 min incubation at 45 °C.
Source: E. coli strain expressing the cloned Taq DNA ligase gene from Thermus aqauticus HB8
Storage Buffer
50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, pH 7.5 @ 25 ºC
10X Taq DNA Ligase reaction buffer with NAD<+
500mM Tris-HCl, 100mM MgCl2, 100mM DTT, 10mM NAD+, pH 7.5 @ 25ºC
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.