• object(anvilstl\commercio\models\Category)#394 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Dna-Protien-Electrophoresis.jpg" ["medium"]=> string(97) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Dna-Protien-Electrophoresis.jpg" ["large"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Dna-Protien-Electrophoresis.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Dna-Protien-Electrophoresis.jpg" ["medium"]=> string(97) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Dna-Protien-Electrophoresis.jpg" ["large"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Dna-Protien-Electrophoresis.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(198) ["root"]=> int(393) ["lft"]=> int(40) ["rgt"]=> int(45) ["level"]=> int(1) ["name"]=> string(35) "DNA & Protein Electrophoresis (old)" ["url_name"]=> string(27) "dna-protein-electrophoresis" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(439) "

    Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.

    " ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(30) ["banner_image_id"]=> int(0) ["meta_description"]=> string(150) "DNA and protein tools are vital to molecular research. GoldBio offers ready-to-use ladders, lysis buffers, Coomassie stains and other needed products." ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(9) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> string(22) "rgba(221, 187, 121, 1)" } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(198) ["root"]=> int(393) ["lft"]=> int(40) ["rgt"]=> int(45) ["level"]=> int(1) ["name"]=> string(35) "DNA & Protein Electrophoresis (old)" ["url_name"]=> string(27) "dna-protein-electrophoresis" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(439) "

    Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.

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  • object(anvilstl\commercio\models\Category)#421 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(86) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Cloning-Induction.jpg" ["medium"]=> string(87) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Cloning-Induction.jpg" ["large"]=> string(86) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Cloning-Induction.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(86) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Cloning-Induction.jpg" ["medium"]=> string(87) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Cloning-Induction.jpg" ["large"]=> string(86) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Cloning-Induction.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(202) ["root"]=> int(393) ["lft"]=> int(56) ["rgt"]=> int(57) ["level"]=> int(1) ["name"]=> string(25) "Cloning & Induction (old)" ["url_name"]=> string(18) "cloning-induction2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(472) "

    Gold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.

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    Gold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.

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  • object(anvilstl\commercio\models\Category)#423 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(353) ["root"]=> int(1) ["lft"]=> int(236) ["rgt"]=> int(237) ["level"]=> int(3) ["name"]=> string(12) "PCR Products" ["url_name"]=> string(12) "pcr-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(755) "PCR (Polymerase Chain Reaction) is one of the most valuable, if not THE most valuable, biochemical techniques used in every molecular biology and biochemistry lab. It is the hallmark of our scientific molecular era, utilized for copying, sequencing or quantifying DNA or RNA. PCR is the process of using thermocycling with heat resistant enzymes to quickly and reliably copy strands of DNA. High quality PCR results save hours of needless replication and frustration and high quality ingredients are essential for producing those results. GoldBio offers the best quality ingredients for your PCR in order to get the most reliable, crisp and clear bands on your gel for use in downstream cloning or if you just need to quickly sequence the PCR strand." 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  • object(anvilstl\commercio\models\Category)#425 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(91) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/ABX Petri with letters.jpg" ["medium"]=> string(92) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/ABX Petri with letters.jpg" ["large"]=> string(91) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/ABX Petri with letters.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(91) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/ABX Petri with letters.jpg" ["medium"]=> string(92) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/ABX Petri with letters.jpg" ["large"]=> string(91) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/ABX Petri with letters.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(375) ["root"]=> int(1) ["lft"]=> int(204) ["rgt"]=> int(205) ["level"]=> int(3) ["name"]=> string(15) "Cloning Enzymes" ["url_name"]=> string(15) "cloning-enzymes" ["title"]=> string(0) "" ["short_description"]=> string(135) "

    Page that is linked to DNA amplification page (generated precisely for DNA amplification hub page). Houses our cloning enzymes.

    " ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(38) ["banner_image_id"]=> int(0) ["meta_description"]=> string(143) "GoldBio offers cloning enzymes including Hot Start Pfu DNA polymerases, ligases, T4 Polynucleotide Kinase to help you achieve cloning success." ["meta_keywords"]=> string(169) "cloning, DNA, enzymes, ligase, ligation, protocol, method, molecular cloning, T4 enzyme, DNA polymerase, blue white screening of colonies, antibiotics, ligation protocol" ["root_sort"]=> int(1551193699) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(3) ["items_per_page"]=> int(25) ["color"]=> string(0) "" } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(375) ["root"]=> int(1) ["lft"]=> int(204) ["rgt"]=> int(205) ["level"]=> int(3) ["name"]=> string(15) "Cloning Enzymes" ["url_name"]=> string(15) "cloning-enzymes" ["title"]=> string(0) "" ["short_description"]=> string(135) "

    Page that is linked to DNA amplification page (generated precisely for DNA amplification hub page). Houses our cloning enzymes.

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array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(2) { [0]=> array(7) { ["id"]=> int(6) ["name"]=> string(13) "Content Block" ["slug"]=> string(13) "content-block" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(2) { [0]=> array(21) { ["id"]=> int(41) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [1]=> array(22) { ["id"]=> int(42) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(7) "Content" 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    In molecular cloning, recombined DNA is generated and inserted into a host for replication. This process requires reliable and efficient enzymes as well as high quality reagents. GoldBio offers multiple cloning enzymes including our Hot Start Pfu DNA polymerases, T4 DNA ligases, T4 DNA polymerases, T4 Polynucleotide Kinase (PNK) to help you achieve cloning success. Here you will also find detailed and comprehensive protocols for different stages of the cloning process.

    " } } } [1]=> array(7) { ["id"]=> int(1) ["name"]=> string(6) "Banner" ["slug"]=> string(6) "banner" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(4) { [0]=> array(21) { ["id"]=> int(28) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [1]=> array(21) { ["id"]=> int(29) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> 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["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(4) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["small"]=> string(95) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/small/Protocols - NW.png" ["medium"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/medium/Protocols - NW.png" ["large"]=> string(95) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/Protocols - NW.png" ["original"]=> string(98) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/Protocols - NW.png" } } } } [1]=> array(7) { ["id"]=> int(8) ["name"]=> string(13) "Resource Card" ["slug"]=> string(13) "resource-card" ["description"]=> NULL ["show_in_picker"]=> int(0) ["deleted_on"]=> NULL ["fields"]=> array(4) { [0]=> array(22) { ["id"]=> int(49) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(1) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(21) "Blue-White Screening " } [1]=> array(21) { ["id"]=> int(50) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "TextAreaField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> 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"ImageField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(4) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["small"]=> string(93) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/small/blue white 2.png" ["medium"]=> string(94) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/medium/blue white 2.png" ["large"]=> string(93) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/blue white 2.png" ["original"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/blue white 2.png" } } } } [2]=> array(7) { ["id"]=> int(8) ["name"]=> string(13) "Resource Card" ["slug"]=> string(13) "resource-card" ["description"]=> NULL ["show_in_picker"]=> int(0) 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"https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/GoldBio-6142.jpg" ["original"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/GoldBio-6142.jpg" } } } } } } } } } } } }
  • Taq DNA Ligase

    {{ i.item.name }}

    Product Description

    Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them; therefore, a single-base substitution can be detected. Taq DNA Ligase is active at elevated temperatures (45-70°C).

    Product Includes

    • Taq DNA Ligase
    • 10X Taq DNA Ligase Buffer with NAD+

    Purity: ≥99% (assessed by SDS-PAGE with Coomassie blue staining)

    Concentration: 40 Units/μl

    Unit Definitions: One unit is defined as the amount of Taq DNA Ligase required to join 50% of 1 μg of the 12-base cohesive ends of Lambda DNA cut with Sma I and Sal I in 50 μl reaction in 15 min incubation at 45 °C.

    Source: E. coli strain expressing the cloned Taq DNA ligase gene from Thermus aqauticus HB8

    Storage Buffer
    50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, pH 7.5 @ 25 ºC

    10X Taq DNA Ligase reaction buffer with NAD<+
    500mM Tris-HCl, 100mM MgCl2, 100mM DTT, 10mM NAD+, pH 7.5 @ 25ºC

    Storage/Handling: Store at -20°C.
    Product may be shipped on blue ice.

    View Sizes & Pricing

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    CAS Number:
    {{ casNumber && casNumber.value ? casNumber.value : '...' }}
    Size:
    Quantity:
    On Sale:
    ${{ currentSelection.price }} ${{ currentSelection.sale_price }}
    Shipping:
    Next day shipping required.

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