object(anvilstl\commercio\models\Category)#394 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(230) ["root"]=> int(1) ["lft"]=> int(27) ["rgt"]=> int(28) ["level"]=> int(2) ["name"]=> string(10) "O_Products" ["url_name"]=> string(10) "o-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(230) ["root"]=> int(1) ["lft"]=> int(27) ["rgt"]=> 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object(anvilstl\commercio\models\Category)#421 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(232) ["root"]=> int(1) ["lft"]=> int(33) ["rgt"]=> int(34) ["level"]=> int(2) ["name"]=> string(10) "R_Products" ["url_name"]=> string(10) "r-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(232) ["root"]=> int(1) ["lft"]=> int(33) ["rgt"]=> 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object(anvilstl\commercio\models\Category)#423 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(233) ["root"]=> int(1) ["lft"]=> int(35) ["rgt"]=> int(36) ["level"]=> int(2) ["name"]=> string(10) "S_Products" ["url_name"]=> string(10) "s-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(233) ["root"]=> int(1) ["lft"]=> int(35) ["rgt"]=> 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object(creocoder\nestedsets\NestedSetsBehavior)#424 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeInsert" } [1]=> NULL } } ["afterInsert"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#424 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL 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object(anvilstl\commercio\models\Category)#425 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(353) ["root"]=> int(1) ["lft"]=> int(236) ["rgt"]=> int(237) ["level"]=> int(3) ["name"]=> string(12) "PCR Products" ["url_name"]=> string(12) "pcr-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(755) "PCR (Polymerase Chain Reaction) is one of the most valuable, if not THE most valuable, biochemical techniques used in every molecular biology and biochemistry lab. It is the hallmark of our scientific molecular era, utilized for copying, sequencing or quantifying DNA or RNA. PCR is the process of using thermocycling with heat resistant enzymes to quickly and reliably copy strands of DNA. High quality PCR results save hours of needless replication and frustration and high quality ingredients are essential for producing those results. GoldBio offers the best quality ingredients for your PCR in order to get the most reliable, crisp and clear bands on your gel for use in downstream cloning or if you just need to quickly sequence the PCR strand." 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PCR is the process of using thermocycling with heat resistant enzymes to quickly and reliably copy strands of DNA. High quality PCR results save hours of needless replication and frustration and high quality ingredients are essential for producing those results. GoldBio offers the best quality ingredients for your PCR in order to get the most reliable, crisp and clear bands on your gel for use in downstream cloning or if you just need to quickly sequence the PCR strand." 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GoldBio has an assortment of kits for RT-PCR, QPCR, and RT-QPCR to maximize and provide consistent and robust transcriptions and amplifications.

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GoldBio has an assortment of kits for RT-PCR, QPCR, and RT-QPCR to maximize and provide consistent and robust transcriptions and amplifications. Our polymerase offers the highest fidelity and best amplification available.

GoldBio has an assortment of kits for RT-PCR, QPCR, and RT-QPCR to maximize and provide consistent and robust transcriptions and amplifications.

" ["long_description"]=> string(229) "

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One Step RT-qPCR kit for SARSCoV-2 (COVID-19) Detection

Name: One Step RT-qPCR kit for SARSCoV-2 (COVID-19) Detection
SKU: P-065
Availability: InStock

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Product Description

This kit is intended for research use only. It is not to be used for diagnostic purposes.

The One Step RT-qPCR kit is used for detection of SARS-CoV-2 in vitro. The approach used for detection is by Real-Time quantitative PCR (RT-qPCR). SARS-CoV-2 is a novel coronavirus identified as the causing agent of a pneumonia outbreak in 2020.

The SARS-CoV-2 detection kit allows cDNA synthesis and qPCR in a single tube. This probe is based on a one step RT-qPCRS 2x master mix, consisting of Reverse Transcriptase, Taq DNA Polymerase, RNase Inhibitor, MgCl₂, dNTPs, stabilizers, and low ROX reference dye with proprietary buffer. The kit provides an improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. In addition, the kit contains CDC recommended primers/probe sets for SARS-CoV-2. This kit can be used to detect SARS-CoV-2 in respiratory specimens; such as sputum, nasopharyngeal, orophayringel aspirates, washes or swabs and tracheal aspirates.

The reverse transcriptase included in the kit is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase included in the kit is a thermostable DNA polymerase that possesses a 5’→3’ polymerase and a 5’→3’ exonuclease activity. The amplification step features a high quality Taq DNA Polymerase with robust, reliable, and better amplification.

The CDC recommended probes included in this One Step RT-qPCR detection kit for SARS-CoV-2 are: 2019-nCoV_N1 Probe, 2019-nCoV_N2 Probe and RNAse P Probe. TaqMan® probes are labeled at the 5′-end with the reporter molecule 6-carboxyfluorescein (FAM) and with the quencher, Black Hole Quencher 1 (BHQ-1) (Biosearch Technologies, Inc., Novato, CA) at the 3′-end.

The One Step RT-qPCR kit is designed to be uses in a 20 μl reaction volume.

Product Includes:

RT-qPCR 2x master mix
CDC recommended primer/probe sets
COVID-19 positive control (PTC)

Benefits

Enhanced efficiency, specificity, and sensitivity
Compatible with all real-time PCR instruments

Storage/Handling
Store the kit at -20°C. The qPCR Master Mix will be shipped on blue ice and should be stored at -20°C immediately upon arrival. When stored under the recommended condition and handled correctly, the kit should be stable for at least 1 year from the date of receipt. Before use, thaw at room temperature and mix well by gentle vortexing. After thawing, the master mix should be kept on ice before use.

Product Specifications

Catalog ID	P-065
Storage/Handling	Store the kit at -20°C.

Technical Documentation

P Protocol

O Other

C Certificate of Analysis

S Safety Data Sheet

One Step RT-qPCR protocol for SARS-COV-2(COVID-19) Detection

Procedure for using Master Mix in the one-step RT-qPCR kit

SDS (MSDS) - One Step RT-qPCR kit for SARSCoV-2 (COVID-19) Detection

Safety Data Sheet

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