i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix

Product Description

i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix is ready to use premix which contains hot start i7® high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® Hot Start High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. This 2X master mix product is supplied with 5X GC enhancer that enables efficient amplification of GC rich templates up to 84%.


  • Long and difficult template DNA amplification
  • Cloning
  • High-fidelity PCR
  • Efficient for amplifying high GC content template DNA with GC enhancer.

Product Includes

  • i7® Hot Start High-Fidelity DNA Polymerase 2x master mix
  • 5X GC Enhancer

Activity Data
Hot start i7® High-Fidelity 2x Master Mix has been tested with difficult templates for PCR amplification. Typical PCR results are shown below:

Hot Start i7 High-Fidelity DNA Polymerase 2x Master Mix Difficult Templates

Storage Buffer:
50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, pH 7.5 @ 25°C

Heat Inactivation: No

Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.

Technical Documentation

P Protocol
O Other
C Certificate of Analysis
S Safety Data Sheet

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