Aminoethyl (High Density) 4% Agarose Beads, Crosslinked
High Density Aminoethyl 4% crosslinked beads allows covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in batch or column purifications (Low Pressure). The resin has a high amount of aldehyde groups (40-60 µm diaminoethyl / mL gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand. The backbone of the resin is 4% agarose beads and the pore size is adequate to work with enzymes, proteins & antibodies.
Aminoethyl resins give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.
Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.
The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/orientation required for the binding to the support.
Aminoethyl 4% agarose beads are suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.
Storage/Handling: Store at 4°C. Do not freeze.
|Storage/Handling||store at 4°C. Do NOT freeze.|
Procedure for Enzyme & Antibody Immobilization onto aminoethyl agarose beads
Technical Specifications - Aminoethyl (High Density) 4% Agarose Beads, Crosslinked
Glyoxal Agarose Resin compared with traditional CNBr activated agarose resin
A Comparison Guide to product similarity between CNBr-activated resins and GoldBio's Aminoethyl/Glyoxal-activated resins
General information about Aminoethyl/Glyoxal Agarose Beads