Ponceau S is the sodium salt of a diazo dye used as a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF. It is often used in the staining of polypeptides to be later blot-sequenced because it does not adversely affect the sequencing of the blotted peptides. The stain is also reversible with water washes, preferable for immunological detection, and can even be completely removed. Ponceau S is a negatively charged stain which binds to positively charged amino acids as well as non-polar regions of proteins. The limit of detection for Ponceau S is ~250 ng of protein.
MOLECULAR BIOLOGY GRADE
MW: 760.57 g/mol
Storage/Handling: Store desiccated at room temperature.
PubChem Chemical ID: 2723873
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store desiccated at room temperature.|