• object(anvilstl\commercio\models\Category)#413 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(99) ["root"]=> int(1) ["lft"]=> int(244) ["rgt"]=> int(245) ["level"]=> int(3) ["name"]=> string(7) "Enzymes" ["url_name"]=> string(7) "enzymes" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(417) "Enzymes assist in carrying out crucial cellular functions, and GoldBio provides a variety of enzymes to accommodate your research needs, including alkaline phosphatase, DNase, RNase, lysozyme, horseradish peroxidase, pepsin and trypsin among others. These products are offered at a lower rate than other commercially available enzymes without sacrificing quality so that researchers attain the highest value possible." 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  • object(anvilstl\commercio\models\Category)#440 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(193) ["root"]=> int(1) ["lft"]=> int(374) ["rgt"]=> int(375) ["level"]=> int(1) ["name"]=> string(17) "Featured Products" ["url_name"]=> string(17) "featured-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(283) "

    Our most popular products from our most popular categories, from our Proven and Published Luciferin to our popular antibiotic lines and electrophoresis tools. GoldBio’s commitment to quality and affordability makes these products our top sellers.

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    Our most popular products from our most popular categories, from our Proven and Published Luciferin to our popular antibiotic lines and electrophoresis tools. GoldBio’s commitment to quality and affordability makes these products our top sellers.

    " ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(98) "GoldBio’s most popular products, made to the highest standards and delivered at the lowest cost." 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  • object(anvilstl\commercio\models\Category)#442 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(200) ["root"]=> int(393) ["lft"]=> int(50) ["rgt"]=> int(51) ["level"]=> int(1) ["name"]=> string(38) "Enzymes, Inhibitors & Substrates (old)" ["url_name"]=> string(30) "enzymes-inhibitors-substrates2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(675) "

    Enzymes are important biological macromolecules that allow for the control of cellular functions and are vital for effective research. Enzyme activity can be slowed or stopped using inhibitors, like Proteinase K or RNase, while substrates like X-Gluc and X-Gal are used in detection and monitoring. D-Luciferin Potassium Salt, our most popular bioluminescent substrate, can be used for in vivo and in vitro imaging. GoldBio knows that having high quality enzyme tools are important to your laboratory, and we are committed to delivering quality products at a fraction of the price.

    " ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(156) "GoldBio carries a huge variety of quality enzymes, inhibitors and substrates, like luciferin, X-Gal and many more to perfectly suit for your research needs." ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(200) ["root"]=> int(393) ["lft"]=> int(50) ["rgt"]=> int(51) ["level"]=> int(1) ["name"]=> string(38) "Enzymes, Inhibitors & Substrates (old)" ["url_name"]=> string(30) "enzymes-inhibitors-substrates2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(675) "

    Enzymes are important biological macromolecules that allow for the control of cellular functions and are vital for effective research. Enzyme activity can be slowed or stopped using inhibitors, like Proteinase K or RNase, while substrates like X-Gluc and X-Gal are used in detection and monitoring. D-Luciferin Potassium Salt, our most popular bioluminescent substrate, can be used for in vivo and in vitro imaging. GoldBio knows that having high quality enzyme tools are important to your laboratory, and we are committed to delivering quality products at a fraction of the price.

    " ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(156) "GoldBio carries a huge variety of quality enzymes, inhibitors and substrates, like luciferin, X-Gal and many more to perfectly suit for your research needs." 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["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeDelete" } [1]=> NULL } } ["afterDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#443 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#443 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }
  • object(anvilstl\commercio\models\Category)#444 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(231) ["root"]=> int(1) ["lft"]=> int(29) ["rgt"]=> int(30) ["level"]=> int(2) ["name"]=> string(10) "P_Products" ["url_name"]=> string(10) "p-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(231) ["root"]=> int(1) ["lft"]=> int(29) ["rgt"]=> int(30) ["level"]=> int(2) ["name"]=> string(10) "P_Products" ["url_name"]=> string(10) "p-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_related":"yii\db\BaseActiveRecord":private]=> array(0) { } ["_relationsDependencies":"yii\db\BaseActiveRecord":private]=> array(0) { } ["_errors":"yii\base\Model":private]=> NULL ["_validators":"yii\base\Model":private]=> NULL ["_scenario":"yii\base\Model":private]=> string(7) "default" ["_events":"yii\base\Component":private]=> array(6) { ["beforeInsert"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeInsert" } [1]=> NULL } } ["afterInsert"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterInsert" } [1]=> NULL } } ["beforeUpdate"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeUpdate" } [1]=> NULL } } ["afterUpdate"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterUpdate" } [1]=> NULL } } ["beforeDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeDelete" } [1]=> NULL } } ["afterDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#445 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }
  • object(anvilstl\commercio\models\Category)#446 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(287) ["root"]=> int(393) ["lft"]=> int(10) ["rgt"]=> int(15) ["level"]=> int(1) ["name"]=> string(32) "Shop for Featured Products (old)" ["url_name"]=> string(22) "featured-products-list" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(287) ["root"]=> int(393) ["lft"]=> int(10) ["rgt"]=> int(15) ["level"]=> int(1) ["name"]=> string(32) "Shop for Featured Products (old)" ["url_name"]=> string(22) "featured-products-list" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_related":"yii\db\BaseActiveRecord":private]=> array(0) { } ["_relationsDependencies":"yii\db\BaseActiveRecord":private]=> array(0) { } ["_errors":"yii\base\Model":private]=> NULL ["_validators":"yii\base\Model":private]=> NULL ["_scenario":"yii\base\Model":private]=> string(7) "default" ["_events":"yii\base\Component":private]=> array(6) { ["beforeInsert"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeInsert" } [1]=> NULL } } ["afterInsert"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterInsert" } [1]=> NULL } } ["beforeUpdate"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeUpdate" } [1]=> NULL } } ["afterUpdate"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterUpdate" } [1]=> NULL } } ["beforeDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeDelete" } [1]=> NULL } } ["afterDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }
  • Proteinase K, RNase/DNase free

    Product Description

    Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

    Proteinase K is typically used at a concentration of 50-100 μg/ml. It is active with or without the presence of SDS, urea, EDTA or various metal ions, but the activity of proteinase K can be increased by adding the denaturing agents and the structure of proteinase K can be stabilized by addition of Ca 2+. Proteinase K is inactivated by heating to 95°C for 10 minutes or using an inhibitor such as PMSF, AEBSF or DFP.

    GoldBio's Proteinase K undergoes two sterile filtration steps during the separation and purification processes. The final product is further analyzed by plate culture to ensure the removal of all living organisms was 100% effective.

    Product Specifications
    Proteinase K, RNase/DNase free

    MOLECULAR BIOLOGY GRADE

    MW: 28.5 kDa

    Activity: ≥30 U/mg

    Unit Definition: One unit is defined as the amount of enzyme that will liberate 1.0 μmol tyrosine (Folin-positive amino acid) from casein per minute at 37°C, pH 7.5.

    Source: Yeast cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

    Storage/Handling: Store at -20°C.
    Reconstitute in 50mM Tris-HCl (pH 8.0), 3mM CaCl2.


    Proteinase K Quick Answers:

    How do you inactivate proteinase K?

    Inactivate proteinase K by heating it to 95 ˚C for 10 minutes. You can also permanently inactivate proteinase K by using protease inhibitors such as PMSF and AEBSF (Pefabloc®).


    What is the best temperature for proteinase K activation?

    The best temperature for proteinase K activity is between 50-65 ˚C. Higher temperatures help with protein unfolding, which makes it easier for proteinase K to breakdown proteins.


    What is proteinase K used for?

    Proteinase K is used in applications such as Digestion of unwanted proteins in molecular biology applications

    • Removal of endotoxins bound to cationic proteins such as lysozyme and RNaseA
    • Removal of nucleases for in situ hybridization
    • Prion research with respect to TSE (transmissible spongiform encephalopathies)
    • Protease footprinting
    • Mitochontrial isolation
    • Isolation of genomic DNA
    • Isolation of cytoplasmic RNA
    • Isolation of highly native DNA or RNA


    How do you store proteinase K?

    Aliquot proteinase K stock solution and store at -20 ˚C for up to 1 year.

    Store lyophilized proteinase K powder desiccated at -20 ˚C for up to 2 years.


    How do you dissolve proteinase K?

    Proteinase K is water soluble and it can also be dissolved in Tris or PBS. Dissolving proteinase K with PBS is a little challenging, which may be due to pH. It's best to add proteinase K a little bit at a time while mixing into solution.


    What is the optimal pH when working with proteinase K?

    The optimal pH when working with proteinase K is between 7.5 - 12.0

    Product Specifications

    Catalog ID P-480
    CAS # 39450-01-6
    MW 28.5 kDa
    Grade MOLECULAR BIOLOGY GRADE
    Storage/Handling Store at -20°C.

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