T7 Endonuclease I
T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits is the product of a recombinant gene in E. coli. Double-stranded breaks (DSBs) generated by CRISPR/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. If the mismatched DNA length position is more than 1 bp, T7 endonuclease I can recognize and cleave it. It is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells.
- T7 Endonuclease I
- 10x T7 Endonuclease I reaction buffer
Purity: ≥99% (assessed by SDS-PAGE with Coomassie blue staining)
Source: E. coli BL21 (DE3) pLysS strain expressing T7 Endonuclease I gene.
1x T7 Endonuclease I reaction buffer
10mM Tris-HCL, 50mM KCl ,10mM MgCl2, 1mM DTT, pH 7.5 at 25ºC
50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, pH 7.5 at 25ºC
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.