{"product_id":"fold-assist-chemically-competent-e-coli-cell-bundle","title":"Fold-Assist Chemically Competent E. Coli Cell Bundle","description":"\u003ch3 data-section-id=\"zacffh\" data-start=\"253\" data-end=\"310\"\u003eFold-Assist Chemically Competent E. Coli Cell Bundle\u003c\/h3\u003e\n\u003cp data-end=\"474\" data-start=\"202\"\u003eDesigned to support the expression of challenging recombinant proteins, the Fold-Assist™ Chaperone Competent Cell Bundle features \u003cem data-start=\"1302\" data-end=\"1311\"\u003eE. coli\u003c\/em\u003e BL21 (DE3) strains engineered to promote proper folding through co-expression of specialized molecular chaperone systems.\u003c\/p\u003e\n\u003cp data-end=\"803\" data-start=\"476\"\u003eThis bundle includes five strains, each carrying a distinct Fold-Assist™ chaperone plasmid (pFA series), designed to provide complementary folding environments for difficult targets. These systems correspond to widely used chaperone configurations and include combinations of DnaK\/DnaJ\/GrpE (KJE), GroEL\/ES, and Trigger Factor:\u003c\/p\u003e\n\u003cul data-end=\"1034\" data-start=\"805\"\u003e\n\u003cli data-end=\"868\" data-start=\"805\" data-section-id=\"1yrk1qt\"\u003e\n\u003cp data-end=\"868\" data-start=\"807\"\u003e\u003cstrong data-end=\"822\" data-start=\"807\"\u003epFA-KJE-Gro\u003c\/strong\u003e — combined DnaK\/DnaJ\/GrpE + GroEL\/ES system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"908\" data-start=\"869\" data-section-id=\"19gt6x2\"\u003e\n\u003cp data-end=\"908\" data-start=\"871\"\u003e\u003cstrong data-end=\"882\" data-start=\"871\"\u003epFA-KJE\u003c\/strong\u003e — DnaK\/DnaJ\/GrpE system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"942\" data-start=\"909\" data-section-id=\"g3gmtv\"\u003e\n\u003cp data-end=\"942\" data-start=\"911\"\u003e\u003cstrong data-end=\"922\" data-start=\"911\"\u003epFA-Gro\u003c\/strong\u003e — GroEL\/ES system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"995\" data-start=\"943\" data-section-id=\"uhlxpb\"\u003e\n\u003cp data-end=\"995\" data-start=\"945\"\u003e\u003cstrong data-end=\"958\" data-start=\"945\"\u003epFA-GroTF\u003c\/strong\u003e — GroEL\/ES + Trigger Factor system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"1034\" data-start=\"996\" data-section-id=\"7mgcv8\"\u003e\n\u003cp data-end=\"1034\" data-start=\"998\"\u003e\u003cstrong data-end=\"1008\" data-start=\"998\"\u003epFA-TF\u003c\/strong\u003e — Trigger Factor system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-end=\"1213\" data-start=\"1036\"\u003eEach plasmid has been engineered in a stabilized, low-burden format to support consistent maintenance and reliable co-expression alongside high-level protein production systems.\u003c\/p\u003e\n\u003cp data-end=\"1398\" data-start=\"1215\"\u003eBuilt on the trusted BL21 (DE3) background, these strains enable robust T7-driven expression while minimizing proteolysis, making them ideal for producing soluble, functional protein.\u003c\/p\u003e\n\u003ch2 data-start=\"1131\" data-end=\"1148\"\u003e\u003cstrong data-start=\"1131\" data-end=\"1148\"\u003eKey Features:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli data-end=\"1545\" data-start=\"1426\" data-section-id=\"y8iqnn\"\u003e\n\u003cp data-end=\"1545\" data-start=\"1428\"\u003e\u003cstrong data-end=\"1467\" data-start=\"1428\"\u003eFive Fold-Assist™ chaperone systems\u003c\/strong\u003e: Screen multiple folding pathways to identify optimal expression conditions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"1695\" data-start=\"1546\" data-section-id=\"k2zwjn\"\u003e\n\u003cp data-end=\"1695\" data-start=\"1548\"\u003e\u003cstrong data-end=\"1603\" data-start=\"1548\"\u003eFunctionally aligned with industry-standard systems\u003c\/strong\u003e: Corresponding to common KJE, GroEL\/ES, and Trigger Factor-based chaperone configurations\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"1804\" data-start=\"1696\" data-section-id=\"sg8sem\"\u003e\n\u003cp data-end=\"1804\" data-start=\"1698\"\u003e\u003cstrong data-end=\"1729\" data-start=\"1698\"\u003eEnhanced protein solubility\u003c\/strong\u003e: Reduce inclusion body formation and increase recovery of active protein\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"1921\" data-start=\"1805\" data-section-id=\"1qxrqx\"\u003e\n\u003cp data-end=\"1921\" data-start=\"1807\"\u003e\u003cstrong data-end=\"1835\" data-start=\"1807\"\u003eOptimized plasmid design\u003c\/strong\u003e: Engineered for stable maintenance and reduced cellular burden during co-expression\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"2043\" data-start=\"1922\" data-section-id=\"mdlml4\"\u003e\n\u003cp data-end=\"2043\" data-start=\"1924\"\u003e\u003cstrong data-end=\"1971\" data-start=\"1924\"\u003eCompatible with standard expression vectors\u003c\/strong\u003e: Designed to perform alongside common high-expression plasmid systems\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"2152\" data-start=\"2044\" data-section-id=\"d9rtnk\"\u003e\n\u003cp data-end=\"2152\" data-start=\"2046\"\u003e\u003cstrong data-end=\"2080\" data-start=\"2046\"\u003eInducible chaperone expression\u003c\/strong\u003e: Fine-tune folding support independently of target protein production\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"2152\" data-start=\"2044\" data-section-id=\"d9rtnk\"\u003e\n\u003cstrong\u003eFree of animal derived components and grown with animal free media.\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"1916\" data-end=\"1933\"\u003e\u003cstrong data-start=\"1916\" data-end=\"1933\"\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"1934\" data-end=\"2169\"\u003e\n\u003cli data-section-id=\"1jeylk\" data-start=\"1934\" data-end=\"1991\"\u003e\n\u003cp data-start=\"1936\" data-end=\"1991\"\u003eExpression of insoluble or aggregation-prone proteins\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-section-id=\"13n4aqe\" data-start=\"1992\" data-end=\"2058\"\u003e\n\u003cp data-start=\"1994\" data-end=\"2058\"\u003eProduction of eukaryotic or multi-domain proteins in \u003cem data-start=\"2047\" data-end=\"2056\"\u003eE. coli\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-section-id=\"11vc9xn\" data-start=\"2059\" data-end=\"2110\"\u003e\n\u003cp data-start=\"2061\" data-end=\"2110\"\u003eStructural biology and protein characterization\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-section-id=\"omb0iw\" data-start=\"2111\" data-end=\"2169\"\u003e\n\u003cp data-start=\"2113\" data-end=\"2169\"\u003eOptimization of recombinant protein yield and activity\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2171\" data-end=\"2458\"\u003e\u003cstrong data-start=\"2171\" data-end=\"2194\"\u003eWorkflow Advantage:\u003c\/strong\u003e\u003cbr\u003eEach strain is pre-transformed with a chaperone plasmid, eliminating the need for sequential cloning and strain construction. This enables rapid screening of multiple folding environments using a single transformation step\u003c\/p\u003e\n\u003ch2 data-start=\"2171\" data-end=\"2458\"\u003e\u003cstrong data-start=\"1916\" data-end=\"1933\"\u003eKit Components:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e2x 100µL of each Chaperone Competent E. Coli Cell line (10x tubes in total)\u003c\/li\u003e\n\u003cli\u003e2 x 12mL Recovery Media\u003c\/li\u003e\n\u003cli\u003e1 x 10µL Control Plasmid (\u003ca href=\"https:\/\/goldbio.com\/product\/puc19-plasmid\"\u003epUC19 Control\u003c\/a\u003e, 500 pg\/µL)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong data-start=\"1916\" data-end=\"1933\"\u003eStorage\/Handling:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis product is shipped on dry ice. Chemically Competent \u003ci\u003eE. coli\u003c\/i\u003e cells should be stored at -80°C, pUC19 Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.\u003c\/p\u003e","brand":"GoldBio","offers":[{"title":"10 x 100 μL","offer_id":45625009307705,"sku":"CC-330-10x100","price":149.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/files\/CC-330-Fold-AssistChemicallyCompetentE.ColiCellBundle_7bc7679f-ea25-4104-a365-8922c16e2d3f.jpg?v=1774297474","url":"https:\/\/www.goldbio.com\/products\/fold-assist-chemically-competent-e-coli-cell-bundle","provider":"GoldBio","version":"1.0","type":"link"}