Description
GoldBio’s Gentamicin Sulfate, USP Grade is a broad-spectrum aminoglycoside antibiotic with potent activity against many gram-negative and select gram-positive bacteria.
USP-grade gentamicin sulfate supports reproducibility in research applications requiring consistent antibiotic performance, including tissue culture systems, microbial assays, and biochemical experiments involving bacterial growth suppression.
Gentamicin inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, disrupting translational fidelity and leading to the production of nonfunctional or toxic peptides. Because of its potent activity against many gram-negative and select gram-positive bacteria, gentamicin is frequently used to suppress bacterial contamination in biological systems and maintain contamination-controlled culture conditions.
Gentamicin sulfate is also widely used in bacterial susceptibility testing, microbial physiology studies, and antibiotic resistance research involving aminoglycoside-responsive organisms.
TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LABS
Mechanism
Gentamicin binds irreversibly to the bacterial 30S ribosomal subunit, interfering with proper codon recognition during translation. This interaction ultimately causes mistranslation of mRNA and incorporation of incorrect amino acids into nascent peptide chains, ultimately leading to production of dysfunctional proteins and disruption of bacterial membrane integrity. The resulting defects contribute to bactericidal activity against susceptible microorganisms.
Common Applications
(Click each for more information)
Antimicrobial Susceptibility Testing
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Purpose: To evaluate bacterial susceptibility or resistance to aminoglycoside antibiotics.
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How It Works: Gentamicin is incorporated into susceptibility assays to assess inhibition of bacterial growth.
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Applications: Disk diffusion assays, MIC determination, and antimicrobial resistance profiling.
Jorgensen, J. H., & Ferraro, M. J. (2009). Antimicrobial susceptibility testing: A review of general principles and contemporary practices. Clinical Infectious Diseases, 49(11), 1749–1755.
Microbiology and Bacterial Physiology Research
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Purpose: To investigate bacterial responses to aminoglycoside exposure.
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How It Works: Gentamicin-induced translational errors alter bacterial viability and physiology.
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Applications: Bacterial growth studies, translational fidelity research, and microbial stress response analysis.
Davis, B. D. (1987). Mechanism of bactericidal action of aminoglycosides. Microbiological Reviews, 51(3), 341–350.
Selection and Maintenance of Contamination-Controlled Cultures
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Purpose: To reduce bacterial contamination in mixed biological preparations.
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How It Works: Gentamicin suppresses susceptible bacterial contaminants while supporting maintenance of experimental systems.
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Applications: Tissue culture maintenance, organoid systems, and primary cell preparation.
Lincoln, C. K., & Gabridge, M. G. (1998). Cell culture contamination: Sources, consequences, prevention, and elimination. Methods in Cell Biology, 57, 49–65.
Aminoglycoside Resistance Research
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Purpose: To study mechanisms of bacterial resistance to aminoglycoside antibiotics.
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How It Works: Researchers evaluate bacterial survival and resistance pathways under gentamicin exposure conditions.
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Applications: Antibiotic resistance studies, plasmid-mediated resistance analysis, and bacterial genetics research.
Ramirez, M. S., & Tolmasky, M. E. (2010). Aminoglycoside modifying enzymes. Drug Resistance Updates, 13(6), 151–171.
Key Benefits
- Broad-spectrum aminoglycoside antibiotic active against many gram-negative bacteria.
- Compatible with antimicrobial susceptibility testing and microbiology research applications.
- USP-grade material supports reproducibility in research environments.
- Useful for bacterial physiology and antibiotic resistance studies.
Storage/Handling
Store desiccated at -20°C.
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