Wonderland

Ring in the new year with one of the most important reagents in your freezer!

GoldBio has been selling proven & publishedTM IPTG for 30 years, testing its product for consistent
quality and high purity. Our IPTG has been cited in thousands of peer-reviewed articles.

Now you can get even MORE HOLIDAY JOY and save 30% off the following IPTG pack sizes by using the promo code:

"WINTER"

IPTG

Catalog ID Size Pricing
I2481C5 5 g $ 27.00 $ 18.90
I2481C25 25 g $ 115.00 $ 80.50
I2481C50 50 g $ 184.00 $ 128.80
I2481C100 100 g $ 349.00 $ 244.30

Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

Product Specifications

Catalog ID I2481
CAS # 367-93-1
MW 238.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

IPTG is commonly used in cloning procedures that require induction of β-galactosidase and is most often used with X-Gal (Gold Bio #X4281C) or Bluo-Gal (Gold Bio #B-673) for blue/white colony screening or Magenta-Gal (Gold Bio #B-378) for red/white colony screening of bacterial colonies. IPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags). GoldBio IPTG is made from a synthetic, non-animal origin.

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