Combining Antibiotics in Mammalian Cell Culture
In his essay “More is Different,” Nobel laureate Dr. Phillip Anderson cautioned against overly reductionist approaches and advised that the complexity found within each scientific...
Antibiotics
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How and When to Test for Interactions When Combining Antibiotics in Cell Culture
You may have heard the phrase that a team is better (or worse) than the sum of its parts. This phrase points out that social...
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How to Deal with Mycoplasma Contamination in Cell Culture
In the story of cell culture, Mycoplasma is the clear antagonist. These tiny, malleable bacteria lack a rigid cell wall and are notoriously difficult to...
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What is Mycoplasma? Understanding Impact on Cell Culture
When doing cell culture work, the term Mycoplasma has probably popped up. Mycoplasma is a genus of bacteria that lacks a cell wall and is...
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Common Side Effects of Antibiotics in Mammalian Cell Culture
Antibiotics and cell selection agents are used to select for specific cell populations to, for instance, generate a stable cell line or conduct a genetic...
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When to Use Antibiotics in Mammalian Cell Culture
Antibiotics are powerful tools that protect cell culture from contamination and help select for transfected cells. However, antibiotics can also have subtle impacts on resistant...
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How to Make Stock Solutions for Antibiotics and Cell Selection Agents
Antibiotics and cell selection agents are used to isolate cells that contain a particular resistance marker from a mixed population. These powerful reagents are used...
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How to Use a Kill Curve to Optimize Antibiotic and Cell Selection Agent Concentrations
During my undergraduate internship I was making different formulations of insulin nanoparticles that would, in theory, be delivered through an inhaler and into the lungs....
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Choosing Between Cell Selection Agents: Puromycin, Blasticidin, Hygromycin, and G418
The cell selection agents puromycin, blasticidin, hygromycin and G418 kill prokaryotic and eukaryotic cells by inhibiting protein translation. Deciding which selection agent, and how much...
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How the Cell Selection Agents Puromycin, Blasticidin, Hygromycin, and G418 Kill Cells
In the microscopic world, different species of bacteria and fungi are engaged in a never-ending turf war, fighting to defend their territory and to advance...
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How a Microbiome is Ruining your Morning Cuppa Joe
Every morning, millions of people all over the world begin their morning routine with a daily dose of stimulating coffee. But did you know that...
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Attention! Calling for Bacterial Cells to Line up Single File for Analysis!
Sometimes, the smallest of things have the biggest impact on our lives. Bacteria inundate every crevice of every larger crevice of our existence. They sustain...
