How to Make Stock Solutions for Antibiotics and Cell Selection Agents
Antibiotics and cell selection agents are used to isolate cells that contain a particular resistance marker from a mixed population. These powerful reagents are used...
Antibiotics
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How to Use a Kill Curve to Optimize Antibiotic and Cell Selection Agent Concentrations
During my undergraduate internship I was making different formulations of insulin nanoparticles that would, in theory, be delivered through an inhaler and into the lungs....
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Choosing Between Cell Selection Agents: Puromycin, Blasticidin, Hygromycin, and G418
The cell selection agents puromycin, blasticidin, hygromycin and G418 kill prokaryotic and eukaryotic cells by inhibiting protein translation. Deciding which selection agent, and how much...
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How the Cell Selection Agents Puromycin, Blasticidin, Hygromycin, and G418 Kill Cells
In the microscopic world, different species of bacteria and fungi are engaged in a never-ending turf war, fighting to defend their territory and to advance...
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How a Microbiome is Ruining your Morning Cuppa Joe
Every morning, millions of people all over the world begin their morning routine with a daily dose of stimulating coffee. But did you know that...
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Attention! Calling for Bacterial Cells to Line up Single File for Analysis!
Sometimes, the smallest of things have the biggest impact on our lives. Bacteria inundate every crevice of every larger crevice of our existence. They sustain...
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Antibiotics in the Wild
There are a lot of things that humans believe that they have invented or discovered that just isn’t true at all. When our ancestors were...
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Antibiotic Agents from the Amazon!
The search for new antibiotics goes on and the accepted norm seems to be keeping our eyes narrowly focused on the bacteria that pervade our...
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Antibiotic Resistance Inherent in the Soil
All of our naturally derived antibiotics come from the ground, or more specifically the organisms that live in the ground. It’s estimated that there are...
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Antibiotic Fall from Grace
It’s extremely well known that our antibiotics are failing us. Alexander Fleming noticed it from the very beginning. Improper use of antibacterial agents leads to...
