How to Make Stock Solutions for Antibiotics and Cell Selection Agents
Antibiotics and cell selection agents are used to isolate cells that contain a particular resistance marker from a mixed population. These powerful reagents are used...
Articles
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How to Use a Kill Curve to Optimize Antibiotic and Cell Selection Agent Concentrations
During my undergraduate internship I was making different formulations of insulin nanoparticles that would, in theory, be delivered through an inhaler and into the lungs....
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Choosing Between Cell Selection Agents: Puromycin, Blasticidin, Hygromycin, and G418
The cell selection agents puromycin, blasticidin, hygromycin and G418 kill prokaryotic and eukaryotic cells by inhibiting protein translation. Deciding which selection agent, and how much...
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How the Cell Selection Agents Puromycin, Blasticidin, Hygromycin, and G418 Kill Cells
In the microscopic world, different species of bacteria and fungi are engaged in a never-ending turf war, fighting to defend their territory and to advance...
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Why are Magnetic Beads Used for Immunoprecipitation and Pull-Down Experiments?
Regular agarose beads are typically used in a column format to purify molecules and perform immunoprecipitation or pull-down experiments. Magnetic agarose beads are an excellent...
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What are Agarose Beads? A Deep Overview
Do you ever feel like you’re bouncing through life, squeezing through the never-ending to-do items on your list? If so, then you have a good...
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Protein Tags: Tools for Protein Detection, Purification, and More
Do you use an AirTag®, Tile®, or other Bluetooth® tracker to keep tabs on anything? When people use these types of tracking devices, it is...
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What is a Plasmid? A Full Overview of Plasmids in Molecular Biology
Did you ever get caught passing a note in class? Or the more modern-day equivalent, were you ever caught sending a text message in class?...
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Comparing CF® Dyes with Other Fluorophores
Small molecule fluorophores can be attached to biomolecules such as proteins for visualization, tracking, and quantitation in a variety of experiments. There are many small...
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Protocol: Biotinylating Avi-Tagged Proteins During Protein Expression
Introduction Biotinylating avi-tagged proteins while they’re being expressed is a convenient way to specifically biotinylate your protein of interest on just its avi-tag. To do...
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Biotinylating Avi-Tagged Proteins During Cellular Expression
The interaction between the small molecule biotin and the protein streptavidin is incredibly strong and has been co-opted for numerous biotechnological purposes. For example, by...
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How to Biotinylate Proteins for Experiments and Purification
The protein streptavidin clamps onto the small molecule biotin in one of the tightest natural interactions ever discovered. Over the years, the strength of this...
