Nickel Affinity Chromatography - The Complete Overview
Nickel affinity chromatography is a technique used to purify proteins that contain a polyhistidine tag (his-tag). Nickel chromatography is a popular choice for affinity purifications...
Articles
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Best Types of Agarose Gels for DNA Electrophoresis – Buyer’s Guide
The best agarose for DNA gels depends on your application. Agarose LE is ideal for routine electrophoresis. Low melt agarose is best for DNA recovery,...
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How to Make an IPTG Stock Solution
Making IPTG stock solution involves weighing out IPTG powder, pouring it into a conical tube or cylinder, and adding deionized water to the desired volume....
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IPTG Induction vs Auto-Induction
IPTG and auto-induction are two ways to induce protein expression in bacteria. They work similarly, but have different trade-offs in terms of convenience. While IPTG...
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Optimizing Protein Expression with IPTG Induction
The final concentration of IPTG used for induction varies from 0.1 to 1.0 mM, with 0.5 or 1.0 mM most frequently used. For proteins with...
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What Is a His-Tag?
A His-tag is a stretch of 6-10 histidine amino acids in a row that is used for affinity purification, protein detection, and biochemical assays. His-tags...
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How Long Do Competent Cells Last?
Competent cells such as DH5a, DH10B, and BL21 will maintain their transformation efficiency for at least a year with proper storage. It is important to...
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Why Did My Nickel Agarose Beads Lose Their Color?
Ni2+ ions give nickel agarose beads their characteristic blue color. This blue color can fade or disappear completely when loading his-tagged proteins onto the column....
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Why Did My Nickel Agarose Beads Turn Black or Brown?
Nickel agarose beads change from blue to a brown or black color when the nickel ions have been reduced from a Ni2+ to a Ni1+...
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Featuring the Coolest Floating Tube Rack Creations
The GoldBio Floating Tube Rack is one of our more clever giveaways because of the unique purpose it serves. And, with it also being one...
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Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
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How to Strip, Clean, and Recharge Nickel Agarose Beads
Like other resins, preparing nickel agarose beads for reuse involves cleaning with solutions that contain high salt, basic pH, or organic solvents. However, these beads...
