AEBSF is an irreversible serine protease inhibitor. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin, kallikrein and thrombin. It inhibits the active site of the enzyme through acylation. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25 mM. Aqueous solutions are stable between pH 5-6; limited stability above pH 7.5.

Dr. Gold’s Bacterial Protein Extraction Reagent is a powerful, next-generation solution designed for efficient extraction of expressed proteins from bacterial cells—now reengineered for improved performance and cost-effectiveness. Built on the foundation of our trusted Bacterial Cell Lysis Buffer, this ready-to-use formulation offers enhanced yield and reproducibility without the need for harsh mechanical disruption.

Our proprietary blend of buffering agents and dialyzable, non-denaturing detergents ensures gentle but thorough lysis, preserving native protein structure and activity.

The reagent includes lysozyme, nucleases, and a specially optimized salt solution to promote full membrane disruption and maximize the recovery of soluble proteins.