During my undergraduate internship I was making different formulations of insulin nanoparticles that would, in theory, be delivered through an inhaler and into the lungs....
The cell selection agents puromycin, blasticidin, hygromycin and G418 kill prokaryotic and eukaryotic cells by inhibiting protein translation. Deciding which selection agent, and how much...
In the microscopic world, different species of bacteria and fungi are engaged in a never-ending turf war, fighting to defend their territory and to advance...
Regular agarose beads are typically used in a column format to purify molecules and perform immunoprecipitation or pull-down experiments. Magnetic agarose beads are an excellent...
Do you ever feel like you’re bouncing through life, squeezing through the never-ending to-do items on your list? If so, then you have a good...
Do you use an AirTag®, Tile®, or other Bluetooth® tracker to keep tabs on anything? When people use these types of tracking devices, it is...
Did you ever get caught passing a note in class? Or the more modern-day equivalent, were you ever caught sending a text message in class?...
Small molecule fluorophores can be attached to biomolecules such as proteins for visualization, tracking, and quantitation in a variety of experiments. There are many small...
Introduction Biotinylating avi-tagged proteins while they’re being expressed is a convenient way to specifically biotinylate your protein of interest on just its avi-tag. To do...
The interaction between the small molecule biotin and the protein streptavidin is incredibly strong and has been co-opted for numerous biotechnological purposes. For example, by...
The protein streptavidin clamps onto the small molecule biotin in one of the tightest natural interactions ever discovered. Over the years, the strength of this...
Maybe you are about to start working with D-luciferin, and you begin reviewing protocols. But what you’re doing is a little different. Or what you...
