Bursting the Bubble, Slaying the `Rat King’
So, you’ve tricked your favorite E. coli expression strain into making gobs of your protein of interest (POI). The SDS-PAGE gel shows a whopping excess...
Affinity resin
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Antibody Purification with Protein A, G
To coincide with the introduction of our new Protein G antibody purification resin, we thought that we’d do a quick introduction to go over the...
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GST-Tagged Protein Purification
There are a number of great methods in the researcher’s toolbox for protein purification. Along with the ever popular His-Tagged protein purification, there is also...
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His-Tag Metal Affinity Cations: What’s the Difference Again?
Unless you’ve done the equivalent of a post-doc on his-tag protein purification, you have probably always wondered about the various metal cations available and what...
