Competent cells such as DH5a, DH10B, and BL21 will maintain their transformation efficiency for at least a year with proper storage. It is important to...
Troubleshooting Electroporation: Conquer Electroporation & Avoid the Arc
If you're getting an arc during electroporation, you can try desalting your DNA, ice your cuvettes, store cuvettes in the freezer to prevent this, or...