How to Regenerate Glutathione Resin
Glutathione agarose beads are cleaned and regenerated by alternating several column volumes of: 1) 0.1M Tris-HCl pH 8.5, 0.5M NaCl, and 2) 0.1M sodium acetate...
Glutathione
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How to Prepare Glutathione Elution Buffer
To make glutathione elution buffer, add reduced glutathione powder to your buffer at a final concentration of 10 to 20 millimolar (mM) within an hour...
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GST-Tagged Protein Purification
There are a number of great methods in the researcher’s toolbox for protein purification. Along with the ever popular His-Tagged protein purification, there is also...
