GST pull-down assays are used to identify protein-protein interactions. A GST-tagged protein is immobilized on glutathione agarose beads and used to pull down interacting proteins...
GST-, MBP-, and SUMO-tags are three affinity tags that help solubilize recombinant proteins. These solubility tags are complete protein domains, so they are much larger...
There are a number of great methods in the researcher’s toolbox for protein purification. Along with the ever popular His-Tagged protein purification, there is also...
