What Is a His-Tag?
A His-tag is a stretch of 6-10 histidine amino acids in a row that is used for affinity purification, protein detection, and biochemical assays. His-tags...
His tag
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Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
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GST-Tagged Protein Purification
There are a number of great methods in the researcher’s toolbox for protein purification. Along with the ever popular His-Tagged protein purification, there is also...
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His-Tag Metal Affinity Cations: What’s the Difference Again?
Unless you’ve done the equivalent of a post-doc on his-tag protein purification, you have probably always wondered about the various metal cations available and what...
