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Inclusion bodies

  • insoluble protein  inclusion body - thumbnail

    Insoluble Protein Purification: How To Get Proteins Out of Inclusion Bodies?

    Proteins are extracted from inclusion bodies using solubilizing salts like guanidine and reducing agents like TCEP. The extracted proteins are then purified and refolded. Functional...

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  • Rat King and inclusion bodies - Mouse among bottles

    Bursting the Bubble, Slaying the `Rat King’

    So, you’ve tricked your favorite E. coli expression strain into making gobs of your protein of interest (POI). The SDS-PAGE gel shows a whopping excess...

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