A Deep Dive Into Induction with IPTG
IPTG induction is a longstanding technique in molecular biology. In this article, you’ll take a deep dive into this important application. You will learn what...
Katharine martin
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Demystifying Material Grades for Your Laboratory
Whether it’s in a chemical’s title or its description, there is usually a note about a reagent’s grade. If you only use molecular biology grade,...
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Troubleshooting Electroporation: Conquer Electroporation & Avoid the Arc
If you're getting an arc during electroporation, you can try desalting your DNA, ice your cuvettes, store cuvettes in the freezer to prevent this, or...
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How to Really Choose a Cell Line - From the Basics to the Complex Aspects of Deciding on Your Cell Line
It’s time to choose a cell line for your project. Research has led you to a few candidates, but narrowing them down can be difficult...
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Five Human Experiments Leading to Scientific Breakthroughs
While there are many human experiments that lead to breakthroughs, some of which are unethical, extremely famous cases include the works of William Beaumont, George...
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Art and Science: An Eternal Relationship
How is science involved with art? Neither seems to have much directly in common. But one of art’s intimate connections with science is based on...
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Weak PCR Band Results or Smearing - What To Do
Immediate solutions for weak or smearing PCR Bands are to check your DNA template, increase your cycle times, and ensure you're not dealing with degraded...
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Getting No Bands in your PCR Result - Tips to Help
There are several troubleshooting tips when you don't get PCR bands, but some basic steps is to ensure you have an organized master mix, your...
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4 PCR Tips When Encountering Nonspecific Binding:
Four important tips when you encounter nonspecific binding include aliquoting, using negative controls, increasing your annealing temperatures and doing touch-down PCR. But each tip needs...
