Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
Nickel agarose beads
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How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...
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How to Strip, Clean, and Recharge Nickel Agarose Beads
Like other resins, preparing nickel agarose beads for reuse involves cleaning with solutions that contain high salt, basic pH, or organic solvents. However, these beads...
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Understanding Binding Capacity for Nickel Agarose Beads
The reported binding capacity of GoldBio’s nickel agarose beads ranges from 6 to 80 milligrams (mg) of his-tagged protein per milliliter (mL) of resin. The...
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What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
While very similar overall, the fundamental difference between NTA and IDA is that NTA forms 4 bonds with a nickel ion whereas IDA makes 3...
