Why Did My Nickel Agarose Beads Lose Their Color?
Ni2+ ions give nickel agarose beads their characteristic blue color. This blue color can fade or disappear completely when loading his-tagged proteins onto the column....
Nickel beads
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Why Did My Nickel Agarose Beads Turn Black or Brown?
Nickel agarose beads change from blue to a brown or black color when the nickel ions have been reduced from a Ni2+ to a Ni1+...
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How to Strip, Clean, and Recharge Nickel Agarose Beads
Like other resins, preparing nickel agarose beads for reuse involves cleaning with solutions that contain high salt, basic pH, or organic solvents. However, these beads...
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What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
While very similar overall, the fundamental difference between NTA and IDA is that NTA forms 4 bonds with a nickel ion whereas IDA makes 3...
