Why Did My Nickel Agarose Beads Lose Their Color?
Ni2+ ions give nickel agarose beads their characteristic blue color. This blue color can fade or disappear completely when loading his-tagged proteins onto the column....
Nickel ida
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Why Did My Nickel Agarose Beads Turn Black or Brown?
Nickel agarose beads change from blue to a brown or black color when the nickel ions have been reduced from a Ni2+ to a Ni1+...
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What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
While very similar overall, the fundamental difference between NTA and IDA is that NTA forms 4 bonds with a nickel ion whereas IDA makes 3...
