Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
Nickel resin
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How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...
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How to Strip, Clean, and Recharge Nickel Agarose Beads
Like other resins, preparing nickel agarose beads for reuse involves cleaning with solutions that contain high salt, basic pH, or organic solvents. However, these beads...
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Bursting the Bubble, Slaying the `Rat King’
So, you’ve tricked your favorite E. coli expression strain into making gobs of your protein of interest (POI). The SDS-PAGE gel shows a whopping excess...
