Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
Protein purification
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Understanding Binding Capacity for Nickel Agarose Beads
The reported binding capacity of GoldBio’s nickel agarose beads ranges from 6 to 80 milligrams (mg) of his-tagged protein per milliliter (mL) of resin. The...
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What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
While very similar overall, the fundamental difference between NTA and IDA is that NTA forms 4 bonds with a nickel ion whereas IDA makes 3...
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An Overview of Hydrophobic Interaction Chromatography
Have you ever felt as if all of your contributions in the lab go unseen and unappreciated? That if only someone would pay attention, they...
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How to Regenerate Glutathione Resin
Glutathione agarose beads are cleaned and regenerated by alternating several column volumes of: 1) 0.1M Tris-HCl pH 8.5, 0.5M NaCl, and 2) 0.1M sodium acetate...
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Understanding Salting In and Salting Out: Salt as a Protein Purification Tool
Salting in is adding salt to a solution to enhance the solubility of a protein, whereas salting out is adding salt to precipitate a protein....
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GST-Tagged Protein Purification
There are a number of great methods in the researcher’s toolbox for protein purification. Along with the ever popular His-Tagged protein purification, there is also...
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His-Tag Metal Affinity Cations: What’s the Difference Again?
Unless you’ve done the equivalent of a post-doc on his-tag protein purification, you have probably always wondered about the various metal cations available and what...
