Why Did My Nickel Agarose Beads Lose Their Color?
Ni2+ ions give nickel agarose beads their characteristic blue color. This blue color can fade or disappear completely when loading his-tagged proteins onto the column....
Protein purification
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Why Did My Nickel Agarose Beads Turn Black or Brown?
Nickel agarose beads change from blue to a brown or black color when the nickel ions have been reduced from a Ni2+ to a Ni1+...
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Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
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What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
While very similar overall, the fundamental difference between NTA and IDA is that NTA forms 4 bonds with a nickel ion whereas IDA makes 3...
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Understanding Binding Capacity for Nickel Agarose Beads
The reported binding capacity of GoldBio’s nickel agarose beads ranges from 6 to 80 milligrams (mg) of his-tagged protein per milliliter (mL) of resin. The...
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An Overview of Hydrophobic Interaction Chromatography
Have you ever felt as if all of your contributions in the lab go unseen and unappreciated? That if only someone would pay attention, they...
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What is a GST Pull-Down Assay? The Complete Overview with Protocols and Analyses Guides
GST pull-down assays are used to identify protein-protein interactions. A GST-tagged protein is immobilized on glutathione agarose beads and used to pull down interacting proteins...
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How to Regenerate Glutathione Resin
Glutathione agarose beads are cleaned and regenerated by alternating several column volumes of: 1) 0.1M Tris-HCl pH 8.5, 0.5M NaCl, and 2) 0.1M sodium acetate...
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Insoluble Protein Purification: How To Get Proteins Out of Inclusion Bodies?
Proteins are extracted from inclusion bodies using solubilizing salts like guanidine and reducing agents like TCEP. The extracted proteins are then purified and refolded. Functional...
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Understanding Salting In and Salting Out: Salt as a Protein Purification Tool
Salting in is adding salt to a solution to enhance the solubility of a protein, whereas salting out is adding salt to precipitate a protein....
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GST Tag, MBP Tag, and SUMO Tag: 3 Solubility Tags for Protein Affinity Purification
GST-, MBP-, and SUMO-tags are three affinity tags that help solubilize recombinant proteins. These solubility tags are complete protein domains, so they are much larger...
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GST-Tagged Protein Purification
There are a number of great methods in the researcher’s toolbox for protein purification. Along with the ever popular His-Tagged protein purification, there is also...
