Why Are Nickel Agarose Beads Blue?
The characteristic blue color of nickel agarose beads comes from the 2+ oxidation state of the nickel ions. Color is also a useful indicator for...
Simon currie
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How to Optimize Buffer Components for Nickel Agarose Beads
Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...
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How to Strip, Clean, and Recharge Nickel Agarose Beads
Like other resins, preparing nickel agarose beads for reuse involves cleaning with solutions that contain high salt, basic pH, or organic solvents. However, these beads...
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Understanding Binding Capacity for Nickel Agarose Beads
The reported binding capacity of GoldBio’s nickel agarose beads ranges from 6 to 80 milligrams (mg) of his-tagged protein per milliliter (mL) of resin. The...
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What are BL21 Competent Cells?
BL21 competent cells are an E. coli strain that is specifically engineered to excel at recombinant protein expression. There are different types of BL21 to...
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What’s the Difference Between Nickel NTA and Nickel IDA Agarose Beads?
While very similar overall, the fundamental difference between NTA and IDA is that NTA forms 4 bonds with a nickel ion whereas IDA makes 3...
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What Are DH10B Competent Cells Used For?
DH10B are an engineered strain of Escherichia coli (E. coli) that are highly efficient at molecular cloning. DH10B cells are particularly adept at handling large...
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What Are DH5a Competent Cells?
DH5a competent cells are a genetically engineered Escherichia coli (E. coli) strain designed for high-efficiency DNA transformation. DH5a is a go-to strain for DNA cloning, plasmid propagation,...
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An Overview of Hydrophobic Interaction Chromatography
Have you ever felt as if all of your contributions in the lab go unseen and unappreciated? That if only someone would pay attention, they...
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How to Regenerate Glutathione Resin
Glutathione agarose beads are cleaned and regenerated by alternating several column volumes of: 1) 0.1M Tris-HCl pH 8.5, 0.5M NaCl, and 2) 0.1M sodium acetate...
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How to Prepare Glutathione Elution Buffer
To make glutathione elution buffer, add reduced glutathione powder to your buffer at a final concentration of 10 to 20 millimolar (mM) within an hour...
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How to Elute Biotinylated Proteins and Nucleic Acids from Streptavidin Beads
Eluting biotinylated proteins and DNA from streptavidin beads requires harsh conditions like enzymatic digestion, extreme pH, organic solvents, and heat with excess biotin. Deciding which...
