How to choose the right Agrobacterium vector for plant transformation
In cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be...
Articles
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An Introduction to Primary Antibodies and Secondary Antibodies
A good signal detection is necessary for the reproducibility of research findings when a researcher performs protein immunoassays. One certain way to obtain good immunoassay...
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An Overview of Polyclonal and Monoclonal Antibodies: Their Differences and How to Choose
Finding the right antibody for your protein immunoassays can be challenging. An antibody, which works well for an assay, may fail to work in another...
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Uncovering the Mystery Behind Glow-in-Dark Fungi
More than 2000 years ago, Aristotle wondered about “a cold fire,” illuminating from the woods. He referred it as “glowing, rotting tree bark” although the...
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An Overview of Stable and Transient Transformation
Stable and transient plant transformation are both very common methods used in plant research. With stable transformation, the foreign DNA taken up by the host...
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How to Manage a Research Project in Graduate School
Entering graduate school is more than getting a degree after completing your undergraduate and learning a sophisticated technical skill in the lab. When you start...
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Deep Dive Into Plant Transformation: Protoplast-Mediated, Biolistic-Mediated and Agrobacterium-Mediated Gene Transfer
Plant transformation is the process in which a foreign gene is introduced into a plant's genome. The methods researchers use for plant transformation include protoplast-mediated...
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The Recipe for Plants: Strategies for Cell Culture Media Preparation
All plants require nutrients to grow. In addition to oxygen, carbon dioxide, and water, plants require at least 14 mineral elements for adequate nutrition. Nitrogen...
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How to Master Collaboration Skills in the Graduate School
It’s common for researchers to collaborate in science. By doing so, scientists can share expertise, obtain more publications and write a stronger grant proposal (Pain,...
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Troubleshooting: Bacterial Transformation
Bacterial transformation is a routine procedure in molecular biology laboratories. After transforming a ligation reaction into Escherichia coli competent cells and plating the cells, you...
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The Hidden Wood Wide Web of the Forests
Underneath a forest lies a giant social network, although it's unnoticeable to us. Similar to how our internet connects us, this hidden network supports a...
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A Guide to T-DNA Binary Vectors in Plant Transformation
Agrobacterium has the ability to transfer T-DNA, a part of a tumor-inducing plasmid or Ti-plasmid, into the genome of many plants. A T-DNA binary system...
